Pretreatment method for extracting saxitoxin from aquatic products
The invention discloses a pretreatment method for extracting saxitoxin from aquatic products. The pretreatment method comprises the following steps: (1) washing the aquatic products and homogenizing; (2) adding the sample homogenized in the step (1) into a solvent, extracting, and taking supernate,...
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| creator | WANG CAN JIANG DIYAO MEI XIUMING WU XIAOXIAO JI HANXU LI YUFENG XU JINGJING ZHANG CHI |
| description | The invention discloses a pretreatment method for extracting saxitoxin from aquatic products. The pretreatment method comprises the following steps: (1) washing the aquatic products and homogenizing; (2) adding the sample homogenized in the step (1) into a solvent, extracting, and taking supernate, the solvent being an acetonitrile aqueous solution; (3) freezing the supernate in the step (2), then taking the supernate, and removing the solvent for detection; the saxitoxin is fat-soluble saxitoxin okadaic acid, a spiral imine compound, dinocystin 1, dinocystin 2 and/or propolydinocystin 1; the method is simple in step, small in organic reagent dosage, low in detection cost and suitable for pretreatment of large-batch samples.
本发明公开了一种提取水产品中贝类毒素的前处理方法，包括以下步骤：(1)将水产品清洗后均质；(2)取步骤(1)均质后的样品加入溶剂中萃取，取上清液，所述溶剂为乙腈水溶液；(3)将步骤(2)的上清液冷冻，然后取上清液，去除溶剂，待测；所述贝类毒素为脂溶性贝类毒素大田软海绵酸、螺旋形亚胺化合物、鳍藻毒素1、鳍藻毒素2和/或原多甲藻酸毒素1；该方法步骤简单，有机试剂用量少，检测成本低，适用于大批量样品前处理。 |
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本发明公开了一种提取水产品中贝类毒素的前处理方法，包括以下步骤：(1)将水产品清洗后均质；(2)取步骤(1)均质后的样品加入溶剂中萃取，取上清液，所述溶剂为乙腈水溶液；(3)将步骤(2)的上清液冷冻，然后取上清液，去除溶剂，待测；所述贝类毒素为脂溶性贝类毒素大田软海绵酸、螺旋形亚胺化合物、鳍藻毒素1、鳍藻毒素2和/或原多甲藻酸毒素1；该方法步骤简单，有机试剂用量少，检测成本低，适用于大批量样品前处理。</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240503&DB=EPODOC&CC=CN&NR=117969726A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25566,76549</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240503&DB=EPODOC&CC=CN&NR=117969726A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG CAN</creatorcontrib><creatorcontrib>JIANG DIYAO</creatorcontrib><creatorcontrib>MEI XIUMING</creatorcontrib><creatorcontrib>WU XIAOXIAO</creatorcontrib><creatorcontrib>JI HANXU</creatorcontrib><creatorcontrib>LI YUFENG</creatorcontrib><creatorcontrib>XU JINGJING</creatorcontrib><creatorcontrib>ZHANG CHI</creatorcontrib><title>Pretreatment method for extracting saxitoxin from aquatic products</title><description>The invention discloses a pretreatment method for extracting saxitoxin from aquatic products. The pretreatment method comprises the following steps: (1) washing the aquatic products and homogenizing; (2) adding the sample homogenized in the step (1) into a solvent, extracting, and taking supernate, the solvent being an acetonitrile aqueous solution; (3) freezing the supernate in the step (2), then taking the supernate, and removing the solvent for detection; the saxitoxin is fat-soluble saxitoxin okadaic acid, a spiral imine compound, dinocystin 1, dinocystin 2 and/or propolydinocystin 1; the method is simple in step, small in organic reagent dosage, low in detection cost and suitable for pretreatment of large-batch samples.
本发明公开了一种提取水产品中贝类毒素的前处理方法，包括以下步骤：(1)将水产品清洗后均质；(2)取步骤(1)均质后的样品加入溶剂中萃取，取上清液，所述溶剂为乙腈水溶液；(3)将步骤(2)的上清液冷冻，然后取上清液，去除溶剂，待测；所述贝类毒素为脂溶性贝类毒素大田软海绵酸、螺旋形亚胺化合物、鳍藻毒素1、鳍藻毒素2和/或原多甲藻酸毒素1；该方法步骤简单，有机试剂用量少，检测成本低，适用于大批量样品前处理。</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEKwjAQANAsDqL-w_kBDq3Q0tEWxUk6uJcjvWigycXLFfL5Ln6A01ve1vSjkAqhBooKgfTNMzgWoKKCVn18QcbilYuP4IQD4GdF9RaS8LxazXuzcbhkOvzcmePt-hzuJ0o8UU5oKZJOw6Oq2q7p2rq5nP85X7R3Mxw</recordid><startdate>20240503</startdate><enddate>20240503</enddate><creator>WANG CAN</creator><creator>JIANG DIYAO</creator><creator>MEI XIUMING</creator><creator>WU XIAOXIAO</creator><creator>JI HANXU</creator><creator>LI YUFENG</creator><creator>XU JINGJING</creator><creator>ZHANG CHI</creator><scope>EVB</scope></search><sort><creationdate>20240503</creationdate><title>Pretreatment method for extracting saxitoxin from aquatic products</title><author>WANG CAN ; JIANG DIYAO ; MEI XIUMING ; WU XIAOXIAO ; JI HANXU ; LI YUFENG ; XU JINGJING ; ZHANG CHI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117969726A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG CAN</creatorcontrib><creatorcontrib>JIANG DIYAO</creatorcontrib><creatorcontrib>MEI XIUMING</creatorcontrib><creatorcontrib>WU XIAOXIAO</creatorcontrib><creatorcontrib>JI HANXU</creatorcontrib><creatorcontrib>LI YUFENG</creatorcontrib><creatorcontrib>XU JINGJING</creatorcontrib><creatorcontrib>ZHANG CHI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG CAN</au><au>JIANG DIYAO</au><au>MEI XIUMING</au><au>WU XIAOXIAO</au><au>JI HANXU</au><au>LI YUFENG</au><au>XU JINGJING</au><au>ZHANG CHI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Pretreatment method for extracting saxitoxin from aquatic products</title><date>2024-05-03</date><risdate>2024</risdate><abstract>The invention discloses a pretreatment method for extracting saxitoxin from aquatic products. The pretreatment method comprises the following steps: (1) washing the aquatic products and homogenizing; (2) adding the sample homogenized in the step (1) into a solvent, extracting, and taking supernate, the solvent being an acetonitrile aqueous solution; (3) freezing the supernate in the step (2), then taking the supernate, and removing the solvent for detection; the saxitoxin is fat-soluble saxitoxin okadaic acid, a spiral imine compound, dinocystin 1, dinocystin 2 and/or propolydinocystin 1; the method is simple in step, small in organic reagent dosage, low in detection cost and suitable for pretreatment of large-batch samples.
本发明公开了一种提取水产品中贝类毒素的前处理方法，包括以下步骤：(1)将水产品清洗后均质；(2)取步骤(1)均质后的样品加入溶剂中萃取，取上清液，所述溶剂为乙腈水溶液；(3)将步骤(2)的上清液冷冻，然后取上清液，去除溶剂，待测；所述贝类毒素为脂溶性贝类毒素大田软海绵酸、螺旋形亚胺化合物、鳍藻毒素1、鳍藻毒素2和/或原多甲藻酸毒素1；该方法步骤简单，有机试剂用量少，检测成本低，适用于大批量样品前处理。</abstract><oa>free_for_read</oa></addata></record> |
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| title | Pretreatment method for extracting saxitoxin from aquatic products |
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