Method for evaluating potential toxic effect of compound on pancreatic beta cells

The invention relates to a method for evaluating a potential toxic effect of a compound on pancreatic beta cells, and belongs to the technical field of human health risk evaluation. The method comprises the following steps: Q1, cell culture; q2, loading a fluorescent probe; q3, medicine exposure; q4, cell active oxygen level detection; q5, judging whether the compound to be detected has a potential toxic effect on the pancreatic beta cells or not; and Q6, cell apoptosis detection. The level of active oxygen in cells is detected through a high-throughput detection method which is good in specificity, easy to operate and low in cost, the potential toxicity of different compounds to the pancreatic beta cells is determined through cell apoptosis detection, more comprehensive evaluation data is obtained, and more comprehensive and reliable data is provided for human health risk evaluation. 本发明涉及一种评估化合物对胰岛β细胞潜在毒性效应的方法，属于人体健康风险评价技术领域；本发明包括Q1：细胞培养；Q2：装载荧光探针；Q3：药物暴露；Q4：细胞活性氧水平检测；Q5：判断待测化合物是否对胰岛β细胞存在潜在毒性效应；Q6：细胞凋亡检测。本发