Application of horse radish peroxidase in preparation of mildew-removing feed

The invention provides application of horse radish peroxidase in preparation of mildew-removing feed, and belongs to the technical field of feed preparation. The preparation method comprises the following steps: constructing recombinant bacillus subtilis for expressing a horse radish peroxidase mutant, expressing horse radish peroxidase, carrying out ultrasonic cracking on thalli, adding a cracking solution into crushed feed raw materials, uniformly stirring and mixing by using a mixing machine, acting at 30 DEG C for 10 hours to degrade mycotoxin, and granulating by using a granulating machine to prepare the feed. Compared with non-mutated horse radish peroxidase, the activity of the horse radish peroxidase mutant for degrading aflatoxin B1 is improved by 54.79%, and the activity of the horse radish peroxidase mutant for degrading zearalenone is improved by 28.03%. The horse radish peroxidase mutant is applied to feed raw material treatment, mycotoxin can be degraded into low-toxicity and non-toxic products,