Probe for detecting mononucleotide mutation and application thereof
The invention belongs to the technical field of biological detection, and provides a probe for detecting mononucleotide mutation and application thereof. The probe provided by the invention is in a configuration that a DNA tetrahedron is connected with four X-type structures, the tetrahedron structu...
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| creator | HUANG GUANZE ZHONG XINYI QIU HONGZHAO HU SHANWEN ZHONG LINMIN |
| description | The invention belongs to the technical field of biological detection, and provides a probe for detecting mononucleotide mutation and application thereof. The probe provided by the invention is in a configuration that a DNA tetrahedron is connected with four X-type structures, the tetrahedron structure is composed of four nucleic acid chains, and the 3'end of each chain is modified with a fluorophore; each X-type structure is composed of three nucleic acid chains and a tetrahedron 5'end, and a left-up nucleic acid chain is modified with a quenching group; each X-type structure is far away from the nucleic acid chain Right-down of the tetrahedron and is provided with a foothold, target DNA can be recognized and paired with the target DNA, the X-type structures are opened, the nucleic acid chain with a quenching group is released, and a fluorescence signal is recovered. According to the specific value of the initial fluorescence signal to the fluorescence signal after the reaction, the detection of the helicobac |
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The probe provided by the invention is in a configuration that a DNA tetrahedron is connected with four X-type structures, the tetrahedron structure is composed of four nucleic acid chains, and the 3'end of each chain is modified with a fluorophore; each X-type structure is composed of three nucleic acid chains and a tetrahedron 5'end, and a left-up nucleic acid chain is modified with a quenching group; each X-type structure is far away from the nucleic acid chain Right-down of the tetrahedron and is provided with a foothold, target DNA can be recognized and paired with the target DNA, the X-type structures are opened, the nucleic acid chain with a quenching group is released, and a fluorescence signal is recovered. 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The probe provided by the invention is in a configuration that a DNA tetrahedron is connected with four X-type structures, the tetrahedron structure is composed of four nucleic acid chains, and the 3'end of each chain is modified with a fluorophore; each X-type structure is composed of three nucleic acid chains and a tetrahedron 5'end, and a left-up nucleic acid chain is modified with a quenching group; each X-type structure is far away from the nucleic acid chain Right-down of the tetrahedron and is provided with a foothold, target DNA can be recognized and paired with the target DNA, the X-type structures are opened, the nucleic acid chain with a quenching group is released, and a fluorescence signal is recovered. According to the specific value of the initial fluorescence signal to the fluorescence signal after the reaction, the detection of the helicobac</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Probe for detecting mononucleotide mutation and application thereof |
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