Whole-cell biosensor for detecting organic mercury, kit and application
The invention discloses a whole-cell biosensor for detecting organic mercury, a kit and application. The invention provides a DNA molecule with the following elements: a mercury sensing element, a vioABCE gene cluster and an organic mercury lyase element, the mercury sensing element comprises merR g...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a whole-cell biosensor for detecting organic mercury, a kit and application. The invention provides a DNA molecule with the following elements: a mercury sensing element, a vioABCE gene cluster and an organic mercury lyase element, the mercury sensing element comprises merR genes and Pmer which are used for coding mercury ion response transcriptional regulatory factors; the organic mercury lyase element comprises a merB gene which is subjected to constitutive expression and is used for coding organic mercury lyase; the Pmer is a two-way promoter, the merR genome forming expression is started in one direction, and the vioABCE gene cluster expression is started in the other direction; the mercury ion response transcriptional regulatory factor is combined with the Pmer and inhibits the promoter activity of the Pmer to the vioABCE gene cluster. The invention also protects recombinant bacteria with the DNA molecule and application of the recombinant bacteria as a whole-cell biosensor for de |
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