Endonuclease VIII as well as preparation method and application thereof
The invention discloses a preparation method and application of endonuclease VIII. Compared with other codon sequences, the codon-optimized nucleotide sequence for encoding the endonuclease VIII provided by the invention can realize massive soluble expression in an escherichia coli system, keeps ver...
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| creator | ZHU WEIWEI ZHENG SANGSANG HE ZUQIANG WANG JIA LIU WENLI JIANG XIWEN |
| description | The invention discloses a preparation method and application of endonuclease VIII. Compared with other codon sequences, the codon-optimized nucleotide sequence for encoding the endonuclease VIII provided by the invention can realize massive soluble expression in an escherichia coli system, keeps very high biological catalytic activity and is suitable for industrial production.
本申请公开了一种核酸内切酶VIII的制备方法和应用。本申请中提供了经密码子优化的编码核酸内切酶VIII的核苷酸序列，相比其他的密码子序列，其能够在大肠杆菌体系中实现大量可溶表达，且保持了很高的生物催化活性，适宜工业化生产。 |
| format | Patent |
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本申请公开了一种核酸内切酶VIII的制备方法和应用。本申请中提供了经密码子优化的编码核酸内切酶VIII的核苷酸序列，相比其他的密码子序列，其能够在大肠杆菌体系中实现大量可溶表达，且保持了很高的生物催化活性，适宜工业化生产。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240322&DB=EPODOC&CC=CN&NR=117737097A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240322&DB=EPODOC&CC=CN&NR=117737097A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHU WEIWEI</creatorcontrib><creatorcontrib>ZHENG SANGSANG</creatorcontrib><creatorcontrib>HE ZUQIANG</creatorcontrib><creatorcontrib>WANG JIA</creatorcontrib><creatorcontrib>LIU WENLI</creatorcontrib><creatorcontrib>JIANG XIWEN</creatorcontrib><title>Endonuclease VIII as well as preparation method and application thereof</title><description>The invention discloses a preparation method and application of endonuclease VIII. Compared with other codon sequences, the codon-optimized nucleotide sequence for encoding the endonuclease VIII provided by the invention can realize massive soluble expression in an escherichia coli system, keeps very high biological catalytic activity and is suitable for industrial production.
本申请公开了一种核酸内切酶VIII的制备方法和应用。本申请中提供了经密码子优化的编码核酸内切酶VIII的核苷酸序列，相比其他的密码子序列，其能够在大肠杆菌体系中实现大量可溶表达，且保持了很高的生物催化活性，适宜工业化生产。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHB3zUvJzytNzklNLE5VCPP09FRILFYoT83JAdEFRakFiUWJJZn5eQq5qSUZ-SkKiXlAXFCQk5kMES7JSC1KzU_jYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GhubmxuYGluaOxsSoAQArozOf</recordid><startdate>20240322</startdate><enddate>20240322</enddate><creator>ZHU WEIWEI</creator><creator>ZHENG SANGSANG</creator><creator>HE ZUQIANG</creator><creator>WANG JIA</creator><creator>LIU WENLI</creator><creator>JIANG XIWEN</creator><scope>EVB</scope></search><sort><creationdate>20240322</creationdate><title>Endonuclease VIII as well as preparation method and application thereof</title><author>ZHU WEIWEI ; ZHENG SANGSANG ; HE ZUQIANG ; WANG JIA ; LIU WENLI ; JIANG XIWEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117737097A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHU WEIWEI</creatorcontrib><creatorcontrib>ZHENG SANGSANG</creatorcontrib><creatorcontrib>HE ZUQIANG</creatorcontrib><creatorcontrib>WANG JIA</creatorcontrib><creatorcontrib>LIU WENLI</creatorcontrib><creatorcontrib>JIANG XIWEN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHU WEIWEI</au><au>ZHENG SANGSANG</au><au>HE ZUQIANG</au><au>WANG JIA</au><au>LIU WENLI</au><au>JIANG XIWEN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Endonuclease VIII as well as preparation method and application thereof</title><date>2024-03-22</date><risdate>2024</risdate><abstract>The invention discloses a preparation method and application of endonuclease VIII. Compared with other codon sequences, the codon-optimized nucleotide sequence for encoding the endonuclease VIII provided by the invention can realize massive soluble expression in an escherichia coli system, keeps very high biological catalytic activity and is suitable for industrial production.
本申请公开了一种核酸内切酶VIII的制备方法和应用。本申请中提供了经密码子优化的编码核酸内切酶VIII的核苷酸序列，相比其他的密码子序列，其能够在大肠杆菌体系中实现大量可溶表达，且保持了很高的生物催化活性，适宜工业化生产。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Endonuclease VIII as well as preparation method and application thereof |
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