Endonuclease VIII as well as preparation method and application thereof

Endonuclease VIII as well as preparation method and application thereof

The invention discloses a preparation method and application of endonuclease VIII. Compared with other codon sequences, the codon-optimized nucleotide sequence for encoding the endonuclease VIII provided by the invention can realize massive soluble expression in an escherichia coli system, keeps ver...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ZHU WEIWEI, ZHENG SANGSANG, HE ZUQIANG, WANG JIA, LIU WENLI, JIANG XIWEN
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention discloses a preparation method and application of endonuclease VIII. Compared with other codon sequences, the codon-optimized nucleotide sequence for encoding the endonuclease VIII provided by the invention can realize massive soluble expression in an escherichia coli system, keeps very high biological catalytic activity and is suitable for industrial production. 本申请公开了一种核酸内切酶VIII的制备方法和应用。本申请中提供了经密码子优化的编码核酸内切酶VIII的核苷酸序列，相比其他的密码子序列，其能够在大肠杆菌体系中实现大量可溶表达，且保持了很高的生物催化活性，适宜工业化生产。