Method for accurately identifying and evaluating peanut allergenicity based on Ara h 2 IgE epitope

Method for accurately identifying and evaluating peanut allergenicity based on Ara h 2 IgE epitope

The invention discloses a method for accurately identifying and evaluating sensitization of peanuts based on an Ara h 2 IgE epitope. The method comprises the following steps: extracting and purifying Ara h 2; preparation of an Ara h 2 IgE epitope monoclonal antibody; preparing an Ara h 2 IgE epitope...

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Hauptverfasser: SHEN CAIYI, ZHAO YIQIAN, YAN YAN, ZHAO JIANGQIANG, DONG YAPING, YAO YI, HE SHENGFA, WANG JIANBIN, YU JINYU
Format: Patent
Sprache:chi ; eng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator SHEN CAIYI
ZHAO YIQIAN
YAN YAN
ZHAO JIANGQIANG
DONG YAPING
YAO YI
HE SHENGFA
WANG JIANBIN
YU JINYU
description The invention discloses a method for accurately identifying and evaluating sensitization of peanuts based on an Ara h 2 IgE epitope. The method comprises the following steps: extracting and purifying Ara h 2; preparation of an Ara h 2 IgE epitope monoclonal antibody; preparing an Ara h 2 IgE epitope polyclonal antibody; analyzing the recognition capability of the epitope antibody on the Ara h 2 IgE epitope peptide; an IgE epitope monoclonal antibody is used as a capture antibody, an IgE epitope polyclonal antibody is used as a detection antibody, Ara h 2 is used as an antigen, and a sandwich ELISA method standard curve for detecting Ara h 2 is established; pretreating a sample to be detected; detecting the sample; evaluating the sensitization of the peanuts in the sample; and establishing a dose-effect relationship. The antibody prepared by the invention can specifically recognize the IgE epitope of Ara h 2, and the established sandwich ELISA method has the capability of rapidly predicting the sensitization o
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Method for accurately identifying and evaluating peanut allergenicity based on Ara h 2 IgE epitope
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