Method for rapidly detecting drug resistance of helicobacter pylori to clarithromycin and detection kit thereof
The invention discloses a method for rapidly detecting drug resistance of helicobacter pylori to clarithromycin and a detection kit thereof. The invention relates to a specific primer for detecting a clarithromycin-resistant 23S rRNA (ribosomal Ribonucleic Acid) gene mutation gene of H.py lori, and...
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| creator | KUANG ZUPENG WU YUWEI ZHAO XINYU ZHANG JUMEI XIE XINQIANG ZHAO HUI WU QINGPING CHEN HUIYUAN LI YING WU LEI LIANG TINGTING GAO HE |
| description | The invention discloses a method for rapidly detecting drug resistance of helicobacter pylori to clarithromycin and a detection kit thereof. The invention relates to a specific primer for detecting a clarithromycin-resistant 23S rRNA (ribosomal Ribonucleic Acid) gene mutation gene of H.py lori, and the specific primer comprises 23S-4F: 5 '-TTGT AGTGGAGGTGAAAATTCCTC-3' and 23S-4R: 5 '-ACCTTACTACAATAGCACTGCT-3'. The specific primer is used for detecting the clarithromycin-resistant 23S rRNA gene mutation gene of H.py lori and comprises 23S-4F: 5 '-TTGT AGTGGAGGTGAAATTCCTC-3'. According to the scheme, the detection anti-interference capacity is higher, even if base mutation except the 2143 site appears in the amplification range of the 2099-2179 sites of the 23S rRNA gene of the to-be-detected strain, the A2143G mutation can still be stably detected, and the A2143G mutation cannot be detected in the strain according to a traditional probe method.
本发明公开了一种快速检测幽门螺杆菌对克拉霉素耐药性的方法及其检测试剂盒。H.py lori对克拉霉素耐药的23S rRNA基因突变基 |
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本发明公开了一种快速检测幽门螺杆菌对克拉霉素耐药性的方法及其检测试剂盒。H.py lori对克拉霉素耐药的23S rRNA基因突变基</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240315&DB=EPODOC&CC=CN&NR=117701745A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240315&DB=EPODOC&CC=CN&NR=117701745A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KUANG ZUPENG</creatorcontrib><creatorcontrib>WU YUWEI</creatorcontrib><creatorcontrib>ZHAO XINYU</creatorcontrib><creatorcontrib>ZHANG JUMEI</creatorcontrib><creatorcontrib>XIE XINQIANG</creatorcontrib><creatorcontrib>ZHAO HUI</creatorcontrib><creatorcontrib>WU QINGPING</creatorcontrib><creatorcontrib>CHEN HUIYUAN</creatorcontrib><creatorcontrib>LI YING</creatorcontrib><creatorcontrib>WU LEI</creatorcontrib><creatorcontrib>LIANG TINGTING</creatorcontrib><creatorcontrib>GAO HE</creatorcontrib><title>Method for rapidly detecting drug resistance of helicobacter pylori to clarithromycin and detection kit thereof</title><description>The invention discloses a method for rapidly detecting drug resistance of helicobacter pylori to clarithromycin and a detection kit thereof. The invention relates to a specific primer for detecting a clarithromycin-resistant 23S rRNA (ribosomal Ribonucleic Acid) gene mutation gene of H.py lori, and the specific primer comprises 23S-4F: 5 '-TTGT AGTGGAGGTGAAAATTCCTC-3' and 23S-4R: 5 '-ACCTTACTACAATAGCACTGCT-3'. The specific primer is used for detecting the clarithromycin-resistant 23S rRNA gene mutation gene of H.py lori and comprises 23S-4F: 5 '-TTGT AGTGGAGGTGAAATTCCTC-3'. According to the scheme, the detection anti-interference capacity is higher, even if base mutation except the 2143 site appears in the amplification range of the 2099-2179 sites of the 23S rRNA gene of the to-be-detected strain, the A2143G mutation can still be stably detected, and the A2143G mutation cannot be detected in the strain according to a traditional probe method.
本发明公开了一种快速检测幽门螺杆菌对克拉霉素耐药性的方法及其检测试剂盒。H.py lori对克拉霉素耐药的23S rRNA基因突变基</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzDEOwjAMQNEuDAi4gzkAEhWgzqgCscDEXpnEaSxCHDlm6O1ZYGf6y9OfN3Ili-IhiIJiYZ8m8GTkjPMIXt8jKFWuhtkRSIBIiZ080BkplCmJMpiAS6hsUeU1Oc6A2f82kuHJBhZJScKymQVMlVbfLpr1-XTvLxsqMlAt6CiTDf2tbbtu23b7w3H3j_kAXSpDmg</recordid><startdate>20240315</startdate><enddate>20240315</enddate><creator>KUANG ZUPENG</creator><creator>WU YUWEI</creator><creator>ZHAO XINYU</creator><creator>ZHANG JUMEI</creator><creator>XIE XINQIANG</creator><creator>ZHAO HUI</creator><creator>WU QINGPING</creator><creator>CHEN HUIYUAN</creator><creator>LI YING</creator><creator>WU LEI</creator><creator>LIANG TINGTING</creator><creator>GAO HE</creator><scope>EVB</scope></search><sort><creationdate>20240315</creationdate><title>Method for rapidly detecting drug resistance of helicobacter pylori to clarithromycin and detection kit thereof</title><author>KUANG ZUPENG ; WU YUWEI ; ZHAO XINYU ; ZHANG JUMEI ; XIE XINQIANG ; ZHAO HUI ; WU QINGPING ; CHEN HUIYUAN ; LI YING ; WU LEI ; LIANG TINGTING ; GAO HE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117701745A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KUANG ZUPENG</creatorcontrib><creatorcontrib>WU YUWEI</creatorcontrib><creatorcontrib>ZHAO XINYU</creatorcontrib><creatorcontrib>ZHANG JUMEI</creatorcontrib><creatorcontrib>XIE XINQIANG</creatorcontrib><creatorcontrib>ZHAO HUI</creatorcontrib><creatorcontrib>WU QINGPING</creatorcontrib><creatorcontrib>CHEN HUIYUAN</creatorcontrib><creatorcontrib>LI YING</creatorcontrib><creatorcontrib>WU LEI</creatorcontrib><creatorcontrib>LIANG TINGTING</creatorcontrib><creatorcontrib>GAO HE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KUANG ZUPENG</au><au>WU YUWEI</au><au>ZHAO XINYU</au><au>ZHANG JUMEI</au><au>XIE XINQIANG</au><au>ZHAO HUI</au><au>WU QINGPING</au><au>CHEN HUIYUAN</au><au>LI YING</au><au>WU LEI</au><au>LIANG TINGTING</au><au>GAO HE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for rapidly detecting drug resistance of helicobacter pylori to clarithromycin and detection kit thereof</title><date>2024-03-15</date><risdate>2024</risdate><abstract>The invention discloses a method for rapidly detecting drug resistance of helicobacter pylori to clarithromycin and a detection kit thereof. The invention relates to a specific primer for detecting a clarithromycin-resistant 23S rRNA (ribosomal Ribonucleic Acid) gene mutation gene of H.py lori, and the specific primer comprises 23S-4F: 5 '-TTGT AGTGGAGGTGAAAATTCCTC-3' and 23S-4R: 5 '-ACCTTACTACAATAGCACTGCT-3'. The specific primer is used for detecting the clarithromycin-resistant 23S rRNA gene mutation gene of H.py lori and comprises 23S-4F: 5 '-TTGT AGTGGAGGTGAAATTCCTC-3'. According to the scheme, the detection anti-interference capacity is higher, even if base mutation except the 2143 site appears in the amplification range of the 2099-2179 sites of the 23S rRNA gene of the to-be-detected strain, the A2143G mutation can still be stably detected, and the A2143G mutation cannot be detected in the strain according to a traditional probe method.
本发明公开了一种快速检测幽门螺杆菌对克拉霉素耐药性的方法及其检测试剂盒。H.py lori对克拉霉素耐药的23S rRNA基因突变基</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for rapidly detecting drug resistance of helicobacter pylori to clarithromycin and detection kit thereof |
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