Method for regulating high mannose glycotype of antibody in CHO cell, CHO cell and application of CHO cell
The invention discloses a method for regulating and controlling an antibody high mannose glycotype in a CHO cell, the CHO cell and application of the CHO cell. The method for regulating and controlling the high mannose glycotype of the antibody in the CHO cell comprises the step of expressing the an...
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| creator | SHI CHAOQUN CHEN WEIBIN ZHAO PUYA CHENG XINRU XU JIAWEN GAO KAI CHEN JIALE YANG ZHENGJIAO YIN XUEFEI |
| description | The invention discloses a method for regulating and controlling an antibody high mannose glycotype in a CHO cell, the CHO cell and application of the CHO cell. The method for regulating and controlling the high mannose glycotype of the antibody in the CHO cell comprises the step of expressing the antibody and GNT1 in the CHO cell. According to the technical scheme, the GNT1 gene is introduced into the CHO cell through vector construction, the problem that the proportion of the high mannose glycotype of the antibody is high is solved, follow-up complex process exploration is avoided, multiple rounds of screening are avoided, and the cost benefit is increased.
本发明公开了一种CHO细胞中调控抗体高甘露糖糖型的方法、CHO细胞及其应用。其中，CHO细胞中调控抗体高甘露糖糖型的方法包括：在CHO细胞中表达抗体和GNT1。应用本发明的技术方案，通过载体构建带入GNT1基因到CHO细胞，改善抗体高甘露糖糖型占比偏高的问题，避免后续复杂的工艺摸索，避免多轮筛选，增加成本效益。 |
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本发明公开了一种CHO细胞中调控抗体高甘露糖糖型的方法、CHO细胞及其应用。其中，CHO细胞中调控抗体高甘露糖糖型的方法包括：在CHO细胞中表达抗体和GNT1。应用本发明的技术方案，通过载体构建带入GNT1基因到CHO细胞，改善抗体高甘露糖糖型占比偏高的问题，避免后续复杂的工艺摸索，避免多轮筛选，增加成本效益。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240315&DB=EPODOC&CC=CN&NR=117701633A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240315&DB=EPODOC&CC=CN&NR=117701633A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHI CHAOQUN</creatorcontrib><creatorcontrib>CHEN WEIBIN</creatorcontrib><creatorcontrib>ZHAO PUYA</creatorcontrib><creatorcontrib>CHENG XINRU</creatorcontrib><creatorcontrib>XU JIAWEN</creatorcontrib><creatorcontrib>GAO KAI</creatorcontrib><creatorcontrib>CHEN JIALE</creatorcontrib><creatorcontrib>YANG ZHENGJIAO</creatorcontrib><creatorcontrib>YIN XUEFEI</creatorcontrib><title>Method for regulating high mannose glycotype of antibody in CHO cell, CHO cell and application of CHO cell</title><description>The invention discloses a method for regulating and controlling an antibody high mannose glycotype in a CHO cell, the CHO cell and application of the CHO cell. The method for regulating and controlling the high mannose glycotype of the antibody in the CHO cell comprises the step of expressing the antibody and GNT1 in the CHO cell. According to the technical scheme, the GNT1 gene is introduced into the CHO cell through vector construction, the problem that the proportion of the high mannose glycotype of the antibody is high is solved, follow-up complex process exploration is avoided, multiple rounds of screening are avoided, and the cost benefit is increased.
本发明公开了一种CHO细胞中调控抗体高甘露糖糖型的方法、CHO细胞及其应用。其中，CHO细胞中调控抗体高甘露糖糖型的方法包括：在CHO细胞中表达抗体和GNT1。应用本发明的技术方案，通过载体构建带入GNT1基因到CHO细胞，改善抗体高甘露糖糖型占比偏高的问题，避免后续复杂的工艺摸索，避免多轮筛选，增加成本效益。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjLEKwjAURbM4iPoPz13BELBzKUoXdXEvMXlJI_G90MYhf28FdXa6F865dy7uJ8w9W3A8wID-GXUO5KEPvoeHJuIRwcdiOJeEwA405XBjWyAQNO0FDMa4-bUJW9ApxWCmI6b34suWYuZ0HHH1yYVYHw_Xpt1i4g7HpA0S5q45S1lVO7lXqlb_OC-iPj7y</recordid><startdate>20240315</startdate><enddate>20240315</enddate><creator>SHI CHAOQUN</creator><creator>CHEN WEIBIN</creator><creator>ZHAO PUYA</creator><creator>CHENG XINRU</creator><creator>XU JIAWEN</creator><creator>GAO KAI</creator><creator>CHEN JIALE</creator><creator>YANG ZHENGJIAO</creator><creator>YIN XUEFEI</creator><scope>EVB</scope></search><sort><creationdate>20240315</creationdate><title>Method for regulating high mannose glycotype of antibody in CHO cell, CHO cell and application of CHO cell</title><author>SHI CHAOQUN ; CHEN WEIBIN ; ZHAO PUYA ; CHENG XINRU ; XU JIAWEN ; GAO KAI ; CHEN JIALE ; YANG ZHENGJIAO ; YIN XUEFEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117701633A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SHI CHAOQUN</creatorcontrib><creatorcontrib>CHEN WEIBIN</creatorcontrib><creatorcontrib>ZHAO PUYA</creatorcontrib><creatorcontrib>CHENG XINRU</creatorcontrib><creatorcontrib>XU JIAWEN</creatorcontrib><creatorcontrib>GAO KAI</creatorcontrib><creatorcontrib>CHEN JIALE</creatorcontrib><creatorcontrib>YANG ZHENGJIAO</creatorcontrib><creatorcontrib>YIN XUEFEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SHI CHAOQUN</au><au>CHEN WEIBIN</au><au>ZHAO PUYA</au><au>CHENG XINRU</au><au>XU JIAWEN</au><au>GAO KAI</au><au>CHEN JIALE</au><au>YANG ZHENGJIAO</au><au>YIN XUEFEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for regulating high mannose glycotype of antibody in CHO cell, CHO cell and application of CHO cell</title><date>2024-03-15</date><risdate>2024</risdate><abstract>The invention discloses a method for regulating and controlling an antibody high mannose glycotype in a CHO cell, the CHO cell and application of the CHO cell. The method for regulating and controlling the high mannose glycotype of the antibody in the CHO cell comprises the step of expressing the antibody and GNT1 in the CHO cell. According to the technical scheme, the GNT1 gene is introduced into the CHO cell through vector construction, the problem that the proportion of the high mannose glycotype of the antibody is high is solved, follow-up complex process exploration is avoided, multiple rounds of screening are avoided, and the cost benefit is increased.
本发明公开了一种CHO细胞中调控抗体高甘露糖糖型的方法、CHO细胞及其应用。其中，CHO细胞中调控抗体高甘露糖糖型的方法包括：在CHO细胞中表达抗体和GNT1。应用本发明的技术方案，通过载体构建带入GNT1基因到CHO细胞，改善抗体高甘露糖糖型占比偏高的问题，避免后续复杂的工艺摸索，避免多轮筛选，增加成本效益。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for regulating high mannose glycotype of antibody in CHO cell, CHO cell and application of CHO cell |
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