Primer for analyzing and identifying HuS-RNase-1 gene of dragon fruit and application of primer

The invention relates to the field of molecular biology and plant molecular breeding, and particularly provides a primer for analyzing and identifying a dragon fruit HuS-RNase-1 gene and application of the primer, and the specific primer is used for analyzing an amplification result and determining a self-incompatible female determinant HuS-RNase-1 gene of each dragon fruit variety to be detected. According to the present invention, with the primer, the detection of the self-incompatible sample of the pitaya can be specifically achieved, the self-incompatible female determinant S gene detection kit containing the primer can rapidly, simply and rapidly identify the self-incompatible female determinant HuS-RNase-1 gene of the unknown pitaya variety, and the suitable pitaya plant can be configured according to the identification result; and a basis is provided for parent matching in dragon fruit cross breeding. 本发明涉及分子生物学和植物分子育种领域，具体提供用于分析鉴定火龙果HuS-RNase-1基因的引物及其应用，以特异引物去分析扩增结果并确定各个待测火龙果品种的自交不亲和雌性决定因子HuS-RNase-1基