Construction method for simulating influenza A virus combined bacterial infection cell model

The invention provides a construction method of a model for simulating influenza A virus combined bacterial infection cells, which comprises the following steps: firstly, using influenza A virus, namely IAV diluted by 50-200 times, to infect mouse mononuclear macrophage leukemia cells, namely RAW264.7 cells, for 3-6 hours; and after removing the virus, culturing the cells for 12-36 hours by using a virus maintenance solution, and then adding lipopolysaccharide with the concentration of 0.25-4 mu g/ml to culture the cells for 12-36 hours to obtain the cell model. According to the invention, the cell model for simulating influenza A virus combined bacterial infection is successfully constructed. The cell model constructed by the method can be used for large-scale screening of drugs for treating influenza virus combined bacterial infection, and drugs with good treatment effect and good prognosis effect are expected to be screened. 本发明提供一种模拟甲型流感病毒合并细菌感染细胞模型的构建方法，所述方法包括先使用稀释50～200倍的甲型流感病毒即IAV感染小鼠单核巨噬细胞白血病细胞3～6h，所述