Engineering strain of high-yield medium-short chain lactone sophorolipid as well as construction method and application of engineering strain
The invention provides an engineering strain of high-yield medium-short chain lactone sophorolipid as well as a construction method and application of the engineering strain, and relates to the technical field of gene engineering. The starting strain of the engineering strain is Starmeria bombycla,...
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| creator | CHEN WEIZHE LIU JIE ZHANG LIPING CHEN YUE YE CANBIN ZHANG MIMIN |
| description | The invention provides an engineering strain of high-yield medium-short chain lactone sophorolipid as well as a construction method and application of the engineering strain, and relates to the technical field of gene engineering. The starting strain of the engineering strain is Starmeria bombycla, and a lactonase SBLE gene and a sophorolipid transporter MDR gene are inserted into the FADH gene locus of the Starmeria bombycla, so that the FADH gene is knocked out. The engineering strain can be used for producing high-yield medium and short-chain lactone sophorolipid through fermentation, and the medium and short-chain lactone sophorolipid (C6-C12) not only has biological functional activities of the lactone sophorolipid, such as antibiosis and anti-tumor, but also has surface activities of the medium and short-chain sophorolipid, such as better solubility and emulsibility, so that the engineering strain has wide application prospect and economic value. The constructed engineering strain and application provid |
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The starting strain of the engineering strain is Starmeria bombycla, and a lactonase SBLE gene and a sophorolipid transporter MDR gene are inserted into the FADH gene locus of the Starmeria bombycla, so that the FADH gene is knocked out. The engineering strain can be used for producing high-yield medium and short-chain lactone sophorolipid through fermentation, and the medium and short-chain lactone sophorolipid (C6-C12) not only has biological functional activities of the lactone sophorolipid, such as antibiosis and anti-tumor, but also has surface activities of the medium and short-chain sophorolipid, such as better solubility and emulsibility, so that the engineering strain has wide application prospect and economic value. 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The starting strain of the engineering strain is Starmeria bombycla, and a lactonase SBLE gene and a sophorolipid transporter MDR gene are inserted into the FADH gene locus of the Starmeria bombycla, so that the FADH gene is knocked out. The engineering strain can be used for producing high-yield medium and short-chain lactone sophorolipid through fermentation, and the medium and short-chain lactone sophorolipid (C6-C12) not only has biological functional activities of the lactone sophorolipid, such as antibiosis and anti-tumor, but also has surface activities of the medium and short-chain sophorolipid, such as better solubility and emulsibility, so that the engineering strain has wide application prospect and economic value. 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The starting strain of the engineering strain is Starmeria bombycla, and a lactonase SBLE gene and a sophorolipid transporter MDR gene are inserted into the FADH gene locus of the Starmeria bombycla, so that the FADH gene is knocked out. The engineering strain can be used for producing high-yield medium and short-chain lactone sophorolipid through fermentation, and the medium and short-chain lactone sophorolipid (C6-C12) not only has biological functional activities of the lactone sophorolipid, such as antibiosis and anti-tumor, but also has surface activities of the medium and short-chain sophorolipid, such as better solubility and emulsibility, so that the engineering strain has wide application prospect and economic value. The constructed engineering strain and application provid</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ADHESIVES ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS DYES ENZYMOLOGY FATTY ACIDS THEREFROM FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES HYGIENE MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MUTATION OR GENETIC ENGINEERING NATURAL RESINS PAINTS POLISHES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE |
| title | Engineering strain of high-yield medium-short chain lactone sophorolipid as well as construction method and application of engineering strain |
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