Method for evaluating continuous synthesis capability of DNA polymerase and application of method
The invention discloses a method for evaluating the continuous synthesis capacity of DNA polymerase and application of the method. The invention designs a method for rapidly evaluating the continuous synthesis capability of DNA polymerase with strand displacement activity, the quantity of product ss...
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| creator | TANG QIONGWEI HAN HUI YANG YUHONG LIU XIANG LI LAN |
| description | The invention discloses a method for evaluating the continuous synthesis capacity of DNA polymerase and application of the method. The invention designs a method for rapidly evaluating the continuous synthesis capability of DNA polymerase with strand displacement activity, the quantity of product ssDNA and the total number of repetition of a target area are quantified through a quantitative PCR method on the basis of a reaction product obtained through rolling circle amplification by using the DNA polymerase, and finally the number of repetition of a unit module in a single ssDNA is calculated, so that the continuous synthesis capability of the DNA polymerase with strand displacement activity is rapidly evaluated. The method is economical and convenient, the test result can reach the precision of the length of a single template, the amplification efficiency and the length of the amplification product can effectively characterize the strand displacement activity of the polymerase, and the continuous synthesis |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for evaluating continuous synthesis capability of DNA polymerase and application of method |
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