Tissue culture method of ornamental aquatic plant Danish red rose
The invention provides a tissue culture method of ornamental aquatic plant Danish red rose. The tissue culture method comprises the following steps: step 1, preparing an explant; sterile cups for production are selected, seedlings grow robustly and are sterile, and the plant height is 1/2-2/3 of the...
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| creator | LIU HAIYING HAN JINGLING DUAN NAN WANG NAIHONG HUANG DONGHAI |
| description | The invention provides a tissue culture method of ornamental aquatic plant Danish red rose. The tissue culture method comprises the following steps: step 1, preparing an explant; sterile cups for production are selected, seedlings grow robustly and are sterile, and the plant height is 1/2-2/3 of the cup height; and 2, differentiation. After the culture medium, roots and miscellaneous leaves are removed, stems containing 1-2 leaf buds are cut and transferred to a differential culture medium, and differentiated seedlings are obtained; and step 3, proliferation. Transferring the differentiated seedlings to a proliferation culture medium to obtain proliferation seedlings; 4, rooting. Transferring the proliferated seedling single plant to a rooting culture medium to obtain a tissue culture seedling, repeating the step 2 to the step 4 to continuously obtain the tissue culture seedling, or directly culturing aquatic plants. The obtained tissue culture seedlings are high in multiplication coefficient, free of plant d |
| format | Patent |
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The tissue culture method comprises the following steps: step 1, preparing an explant; sterile cups for production are selected, seedlings grow robustly and are sterile, and the plant height is 1/2-2/3 of the cup height; and 2, differentiation. After the culture medium, roots and miscellaneous leaves are removed, stems containing 1-2 leaf buds are cut and transferred to a differential culture medium, and differentiated seedlings are obtained; and step 3, proliferation. Transferring the differentiated seedlings to a proliferation culture medium to obtain proliferation seedlings; 4, rooting. Transferring the proliferated seedling single plant to a rooting culture medium to obtain a tissue culture seedling, repeating the step 2 to the step 4 to continuously obtain the tissue culture seedling, or directly culturing aquatic plants. 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The tissue culture method comprises the following steps: step 1, preparing an explant; sterile cups for production are selected, seedlings grow robustly and are sterile, and the plant height is 1/2-2/3 of the cup height; and 2, differentiation. After the culture medium, roots and miscellaneous leaves are removed, stems containing 1-2 leaf buds are cut and transferred to a differential culture medium, and differentiated seedlings are obtained; and step 3, proliferation. Transferring the differentiated seedlings to a proliferation culture medium to obtain proliferation seedlings; 4, rooting. Transferring the proliferated seedling single plant to a rooting culture medium to obtain a tissue culture seedling, repeating the step 2 to the step 4 to continuously obtain the tissue culture seedling, or directly culturing aquatic plants. The obtained tissue culture seedlings are high in multiplication coefficient, free of plant d</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>TRAPPING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyjEOwjAMBdAsDAi4gzkAQ1SkzFULYmLqXlnprxopTULs3J-FAzC95R1NPwWRBvItaqugHbrlhfJKuSbekZQj8aexBk8lclIaOQXZqGKhmgVnc1g5Ci4_T-b6fEzD64aSZ0hhjwSdh7e17m4751zf_XO-DKcxrg</recordid><startdate>20240119</startdate><enddate>20240119</enddate><creator>LIU HAIYING</creator><creator>HAN JINGLING</creator><creator>DUAN NAN</creator><creator>WANG NAIHONG</creator><creator>HUANG DONGHAI</creator><scope>EVB</scope></search><sort><creationdate>20240119</creationdate><title>Tissue culture method of ornamental aquatic plant Danish red rose</title><author>LIU HAIYING ; HAN JINGLING ; DUAN NAN ; WANG NAIHONG ; HUANG DONGHAI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117413777A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>TRAPPING</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU HAIYING</creatorcontrib><creatorcontrib>HAN JINGLING</creatorcontrib><creatorcontrib>DUAN NAN</creatorcontrib><creatorcontrib>WANG NAIHONG</creatorcontrib><creatorcontrib>HUANG DONGHAI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU HAIYING</au><au>HAN JINGLING</au><au>DUAN NAN</au><au>WANG NAIHONG</au><au>HUANG DONGHAI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Tissue culture method of ornamental aquatic plant Danish red rose</title><date>2024-01-19</date><risdate>2024</risdate><abstract>The invention provides a tissue culture method of ornamental aquatic plant Danish red rose. The tissue culture method comprises the following steps: step 1, preparing an explant; sterile cups for production are selected, seedlings grow robustly and are sterile, and the plant height is 1/2-2/3 of the cup height; and 2, differentiation. After the culture medium, roots and miscellaneous leaves are removed, stems containing 1-2 leaf buds are cut and transferred to a differential culture medium, and differentiated seedlings are obtained; and step 3, proliferation. Transferring the differentiated seedlings to a proliferation culture medium to obtain proliferation seedlings; 4, rooting. Transferring the proliferated seedling single plant to a rooting culture medium to obtain a tissue culture seedling, repeating the step 2 to the step 4 to continuously obtain the tissue culture seedling, or directly culturing aquatic plants. The obtained tissue culture seedlings are high in multiplication coefficient, free of plant d</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
| title | Tissue culture method of ornamental aquatic plant Danish red rose |
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