Fluorescent probe as well as preparation method and application thereof

The invention provides a novel fluorescent probe Nap-NO2 which is used for rapidly and accurately detecting human serum albumin. The preparation method comprises the step of carrying out a reaction on the N-n-butyl-3-formyl-4-hydroxy-1, 8-naphthalimide and a pyridinium salt. The fluorescent probe Nap-NO2 has the advantages of red light emission, quick response, strong specificity and selectivity, low detection limit, good stability, simple and convenient detection process and the like, and has strong application value in the aspects of preparation of detection products of human serum albumin and screening or detection of human serum albumin IB structural domain related drugs. 本发明提供了一种新型的荧光探针Nap-NO2，用于人血清白蛋白的快速准确检测。其制备方法包括N-正丁基-3-醛基-4-羟基-1,8-萘酰亚胺与吡啶盐的反应。荧光探针Nap-NO2具有红光发射、快速响应、专一选择性强、低检测限、稳定性好、检测过程简便等优点，在制备人血清白蛋白的检测产品方面，以及在筛选或检测人血清白蛋白IB结构域相关药物方面，都具有较强的应用价值。