Plant multi-gene editing system and assembling method thereof

The invention discloses a plant multi-gene editing system and an assembling method thereof, and belongs to the technical field of biology. The system comprises eight donor carriers, two template carriers and four accepting carriers. According to the system, a Golden Gate method is utilized to construct entry vectors which have different numbers of sgRNA expression cassettes and contain different Gateway recombinant arms; and finally, assembling different entry vectors onto a receiving vector through a recombination reaction to realize the construction of the multi-gene editing expression vector. According to the invention, a plurality of sgRNA expression cassettes can be assembled to a single expression vector, and the target of multi-target operation genome, including knockout, base editing, transcription inhibition or activation, can be realized. Therefore, the plant editing system is suitable for polygene knockout, polygene high-intensity regulation and control and disaturated single base editing, can be a