Microorganism pretreatment method, nucleic acid extraction method, kit and device
The invention belongs to the technical field of nucleic acid detection, and relates to a microorganism pretreatment method, a nucleic acid extraction method, a kit and a device. The pretreatment method comprises the following steps: carrying out high-temperature short-time pyrolysis treatment on a m...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | JIN LUYI GUO LIPING ZHANG YE CHEN QIMENG XIA HAN ZHANG HUAYANG GUAN YUANLIN |
| description | The invention belongs to the technical field of nucleic acid detection, and relates to a microorganism pretreatment method, a nucleic acid extraction method, a kit and a device. The pretreatment method comprises the following steps: carrying out high-temperature short-time pyrolysis treatment on a microbial sample to be detected under a neutral or weakly alkaline condition, and then carrying out mechanical wall breaking to obtain a sample for nucleic acid extraction and purification. According to the method provided by the invention, the sample for nucleic acid extraction is obtained, the swab sample is fully split, a splitting solution, protease K and the like are not needed for auxiliary splitting, the nucleic acid extraction efficiency is improved, the extraction cost is reduced, and the method is suitable for microorganisms with stubborn cell walls.
本发明属于核酸检测技术领域，涉及微生物的预处理方法、核酸提取方法、试剂盒及装置。该预处理方法采用在中性或弱碱性条件下对待测微生物样本进行高温短时热解处理，然后进行机械破壁，得到用于核酸提取纯化的样本。本发明提供的方法得到用于核酸提取的样本，对拭子样本进行充分的裂解，不需要裂解液和蛋白酶K等进行辅助裂解，提高了核酸提 |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN117327586A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN117327586A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN117327586A3</originalsourceid><addsrcrecordid>eNqNyrEKwkAQRdFtLET9h7HXIgaNrQTFRkGwD8Psiw4mu2F3FD_fRqytbnHu2F1OKinGdOOguachwRLYegSjHnaPfkHhKR1UiEU94W2JxTSGnz_UiIMnj5cKpm7Ucpcx-3bi5of9tT4uMcQGeWBBgDX1uSiqclWtt5td-c_zAQKXN9c</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Microorganism pretreatment method, nucleic acid extraction method, kit and device</title><source>esp@cenet</source><creator>JIN LUYI ; GUO LIPING ; ZHANG YE ; CHEN QIMENG ; XIA HAN ; ZHANG HUAYANG ; GUAN YUANLIN</creator><creatorcontrib>JIN LUYI ; GUO LIPING ; ZHANG YE ; CHEN QIMENG ; XIA HAN ; ZHANG HUAYANG ; GUAN YUANLIN</creatorcontrib><description>The invention belongs to the technical field of nucleic acid detection, and relates to a microorganism pretreatment method, a nucleic acid extraction method, a kit and a device. The pretreatment method comprises the following steps: carrying out high-temperature short-time pyrolysis treatment on a microbial sample to be detected under a neutral or weakly alkaline condition, and then carrying out mechanical wall breaking to obtain a sample for nucleic acid extraction and purification. According to the method provided by the invention, the sample for nucleic acid extraction is obtained, the swab sample is fully split, a splitting solution, protease K and the like are not needed for auxiliary splitting, the nucleic acid extraction efficiency is improved, the extraction cost is reduced, and the method is suitable for microorganisms with stubborn cell walls.
本发明属于核酸检测技术领域，涉及微生物的预处理方法、核酸提取方法、试剂盒及装置。该预处理方法采用在中性或弱碱性条件下对待测微生物样本进行高温短时热解处理，然后进行机械破壁，得到用于核酸提取纯化的样本。本发明提供的方法得到用于核酸提取的样本，对拭子样本进行充分的裂解，不需要裂解液和蛋白酶K等进行辅助裂解，提高了核酸提</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240102&DB=EPODOC&CC=CN&NR=117327586A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240102&DB=EPODOC&CC=CN&NR=117327586A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>JIN LUYI</creatorcontrib><creatorcontrib>GUO LIPING</creatorcontrib><creatorcontrib>ZHANG YE</creatorcontrib><creatorcontrib>CHEN QIMENG</creatorcontrib><creatorcontrib>XIA HAN</creatorcontrib><creatorcontrib>ZHANG HUAYANG</creatorcontrib><creatorcontrib>GUAN YUANLIN</creatorcontrib><title>Microorganism pretreatment method, nucleic acid extraction method, kit and device</title><description>The invention belongs to the technical field of nucleic acid detection, and relates to a microorganism pretreatment method, a nucleic acid extraction method, a kit and a device. The pretreatment method comprises the following steps: carrying out high-temperature short-time pyrolysis treatment on a microbial sample to be detected under a neutral or weakly alkaline condition, and then carrying out mechanical wall breaking to obtain a sample for nucleic acid extraction and purification. According to the method provided by the invention, the sample for nucleic acid extraction is obtained, the swab sample is fully split, a splitting solution, protease K and the like are not needed for auxiliary splitting, the nucleic acid extraction efficiency is improved, the extraction cost is reduced, and the method is suitable for microorganisms with stubborn cell walls.
本发明属于核酸检测技术领域，涉及微生物的预处理方法、核酸提取方法、试剂盒及装置。该预处理方法采用在中性或弱碱性条件下对待测微生物样本进行高温短时热解处理，然后进行机械破壁，得到用于核酸提取纯化的样本。本发明提供的方法得到用于核酸提取的样本，对拭子样本进行充分的裂解，不需要裂解液和蛋白酶K等进行辅助裂解，提高了核酸提</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEKwkAQRdFtLET9h7HXIgaNrQTFRkGwD8Psiw4mu2F3FD_fRqytbnHu2F1OKinGdOOguachwRLYegSjHnaPfkHhKR1UiEU94W2JxTSGnz_UiIMnj5cKpm7Ucpcx-3bi5of9tT4uMcQGeWBBgDX1uSiqclWtt5td-c_zAQKXN9c</recordid><startdate>20240102</startdate><enddate>20240102</enddate><creator>JIN LUYI</creator><creator>GUO LIPING</creator><creator>ZHANG YE</creator><creator>CHEN QIMENG</creator><creator>XIA HAN</creator><creator>ZHANG HUAYANG</creator><creator>GUAN YUANLIN</creator><scope>EVB</scope></search><sort><creationdate>20240102</creationdate><title>Microorganism pretreatment method, nucleic acid extraction method, kit and device</title><author>JIN LUYI ; GUO LIPING ; ZHANG YE ; CHEN QIMENG ; XIA HAN ; ZHANG HUAYANG ; GUAN YUANLIN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117327586A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>JIN LUYI</creatorcontrib><creatorcontrib>GUO LIPING</creatorcontrib><creatorcontrib>ZHANG YE</creatorcontrib><creatorcontrib>CHEN QIMENG</creatorcontrib><creatorcontrib>XIA HAN</creatorcontrib><creatorcontrib>ZHANG HUAYANG</creatorcontrib><creatorcontrib>GUAN YUANLIN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>JIN LUYI</au><au>GUO LIPING</au><au>ZHANG YE</au><au>CHEN QIMENG</au><au>XIA HAN</au><au>ZHANG HUAYANG</au><au>GUAN YUANLIN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Microorganism pretreatment method, nucleic acid extraction method, kit and device</title><date>2024-01-02</date><risdate>2024</risdate><abstract>The invention belongs to the technical field of nucleic acid detection, and relates to a microorganism pretreatment method, a nucleic acid extraction method, a kit and a device. The pretreatment method comprises the following steps: carrying out high-temperature short-time pyrolysis treatment on a microbial sample to be detected under a neutral or weakly alkaline condition, and then carrying out mechanical wall breaking to obtain a sample for nucleic acid extraction and purification. According to the method provided by the invention, the sample for nucleic acid extraction is obtained, the swab sample is fully split, a splitting solution, protease K and the like are not needed for auxiliary splitting, the nucleic acid extraction efficiency is improved, the extraction cost is reduced, and the method is suitable for microorganisms with stubborn cell walls.
本发明属于核酸检测技术领域，涉及微生物的预处理方法、核酸提取方法、试剂盒及装置。该预处理方法采用在中性或弱碱性条件下对待测微生物样本进行高温短时热解处理，然后进行机械破壁，得到用于核酸提取纯化的样本。本发明提供的方法得到用于核酸提取的样本，对拭子样本进行充分的裂解，不需要裂解液和蛋白酶K等进行辅助裂解，提高了核酸提</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN117327586A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Microorganism pretreatment method, nucleic acid extraction method, kit and device |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T17%3A24%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=JIN%20LUYI&rft.date=2024-01-02&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN117327586A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |