Sample diluent, detection kit and method for indirect ELISA detection of African swine fever virus

The invention belongs to the technical field of biological detection, and particularly discloses a sample diluent, a detection kit and a method for indirect ELISA detection of African swine fever virus. The sample diluent for indirect ELISA detection of the African swine fever virus, provided by the invention, contains the escherichia coli prokaryotic expression protein, and can effectively reduce the influence of the background of a serum sample to be detected, block the interference of irrelevant antibodies and improve the specificity and sensitivity of African swine fever virus antibody detection. The African swine fever virus indirect ELISA detection kit provided by the invention can rapidly and accurately detect whether an African swine fever virus antibody exists in a clinical serum sample or not, the coincidence rate with a commercial kit is as high as 96.77% through verification, the sensitivity is higher than that of the commercial kit, the stability is good at 4 DEG C, the storage life can at least