Artificial nano-channel capable of being reversibly switched among different permeation states as well as preparation method and application of artificial nano-channel

The invention provides an artificial nano-channel capable of being reversibly switched among different permeation states, and the artificial nano-channel is constructed by using metal ion specific RNA to cut DNAzymes and a substrate of the DNAzymes as functional molecules to modify a glass nano-pipette. Hydrophilicity and hydrophobicity and charges in the nano channel are reversibly adjusted and controlled through metal ions and corresponding DNAzyme substrate chains, and selective access of substances with different properties is achieved. And the tip nanochannel is inserted into a cell membrane and is used for simulating a biological channel, so that the influence of the surface characteristics of the artificial bionic nanochannel on selective access of substances and the application of the artificial bionic nanochannel are explored. 本发明提供一种能在不同渗透状态间可逆切换的人工纳米通道，使用金属离子特异性RNA切割DNAzymes及其底物作为功能分子来修饰玻璃纳米吸管以构建人工纳米通道，通过金属离子和相应的DNAzyme底物链对纳米通道内的亲疏水性和电荷进行可逆的调节和控制，实现了对不同性质的物质选择性进出。并将尖端纳米通道插在细胞膜上，用于模仿生物通道，探究了人工仿生纳米通道