LAMP (loop-mediated isothermal amplification) detection primer, detection method and kit for Yunnan tobacco leaf curl virus
The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) detection primer, a detection method and a kit for Yunnan tobacco leaf curl virus, and the detection primer is TbL-3, TbL-4 or TbL-5. When the obtained primer is used for detecting the LAMP of the Yunnan tobacco leaf curl virus...
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| creator | LIU YATING ZHANG JUNLEI XIA YUQI LIU DI GE XIAOTONG XIA ZHENYUAN ZHENG XIANTAO ZHAO ZHENGTING JIANG NING |
| description | The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) detection primer, a detection method and a kit for Yunnan tobacco leaf curl virus, and the detection primer is TbL-3, TbL-4 or TbL-5. When the obtained primer is used for detecting the LAMP of the Yunnan tobacco leaf curl virus, the sensitivity is 10000 times that of common PCR, the LAMP amplification reaction time is 60 min, the detection efficiency is high, the obtained result can be visually observed and read out, the detection efficiency is effectively improved, and the primer is especially suitable for detection of large-batch samples.
本申请公开了一种云南烟草曲叶病毒用LAMP检测引物、检测方法及其试剂盒，其中检测引物，为：TbL-3、TbL-4或TbL-5。所得引物用于云南烟草曲叶病毒检测LAMP时，灵敏度是普通PCR的10000倍，LAMP扩增反应时间为60min，检测效率较高，所得结果可直观观察读出，有效提高检测效率，尤其适用于大批量样品的检测。 |
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本申请公开了一种云南烟草曲叶病毒用LAMP检测引物、检测方法及其试剂盒，其中检测引物，为：TbL-3、TbL-4或TbL-5。所得引物用于云南烟草曲叶病毒检测LAMP时，灵敏度是普通PCR的10000倍，LAMP扩增反应时间为60min，检测效率较高，所得结果可直观观察读出，有效提高检测效率，尤其适用于大批量样品的检测。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231205&DB=EPODOC&CC=CN&NR=117165720A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231205&DB=EPODOC&CC=CN&NR=117165720A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU YATING</creatorcontrib><creatorcontrib>ZHANG JUNLEI</creatorcontrib><creatorcontrib>XIA YUQI</creatorcontrib><creatorcontrib>LIU DI</creatorcontrib><creatorcontrib>GE XIAOTONG</creatorcontrib><creatorcontrib>XIA ZHENYUAN</creatorcontrib><creatorcontrib>ZHENG XIANTAO</creatorcontrib><creatorcontrib>ZHAO ZHENGTING</creatorcontrib><creatorcontrib>JIANG NING</creatorcontrib><title>LAMP (loop-mediated isothermal amplification) detection primer, detection method and kit for Yunnan tobacco leaf curl virus</title><description>The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) detection primer, a detection method and a kit for Yunnan tobacco leaf curl virus, and the detection primer is TbL-3, TbL-4 or TbL-5. When the obtained primer is used for detecting the LAMP of the Yunnan tobacco leaf curl virus, the sensitivity is 10000 times that of common PCR, the LAMP amplification reaction time is 60 min, the detection efficiency is high, the obtained result can be visually observed and read out, the detection efficiency is effectively improved, and the primer is especially suitable for detection of large-batch samples.
本申请公开了一种云南烟草曲叶病毒用LAMP检测引物、检测方法及其试剂盒，其中检测引物，为：TbL-3、TbL-4或TbL-5。所得引物用于云南烟草曲叶病毒检测LAMP时，灵敏度是普通PCR的10000倍，LAMP扩增反应时间为60min，检测效率较高，所得结果可直观观察读出，有效提高检测效率，尤其适用于大批量样品的检测。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNirEKwjAQQLs4iPoP56ZgwSrauRTFQcXBxamcyYUGk1xIry7-vAgOjk7v8XjD7HWsTheYOeaYe9IWhTTYjqWl5NEB-uissQrFcpiDJiH1UYjJekqLn-JJWtaAQcPDChhOcOtDwADCd1SKwREaUH1y8LSp78bZwKDraPLlKJvud9f6kFPkhrqIigJJU5-Loiy2m3K1rNb_PG87-0bM</recordid><startdate>20231205</startdate><enddate>20231205</enddate><creator>LIU YATING</creator><creator>ZHANG JUNLEI</creator><creator>XIA YUQI</creator><creator>LIU DI</creator><creator>GE XIAOTONG</creator><creator>XIA ZHENYUAN</creator><creator>ZHENG XIANTAO</creator><creator>ZHAO ZHENGTING</creator><creator>JIANG NING</creator><scope>EVB</scope></search><sort><creationdate>20231205</creationdate><title>LAMP (loop-mediated isothermal amplification) detection primer, detection method and kit for Yunnan tobacco leaf curl virus</title><author>LIU YATING ; ZHANG JUNLEI ; XIA YUQI ; LIU DI ; GE XIAOTONG ; XIA ZHENYUAN ; ZHENG XIANTAO ; ZHAO ZHENGTING ; JIANG NING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117165720A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU YATING</creatorcontrib><creatorcontrib>ZHANG JUNLEI</creatorcontrib><creatorcontrib>XIA YUQI</creatorcontrib><creatorcontrib>LIU DI</creatorcontrib><creatorcontrib>GE XIAOTONG</creatorcontrib><creatorcontrib>XIA ZHENYUAN</creatorcontrib><creatorcontrib>ZHENG XIANTAO</creatorcontrib><creatorcontrib>ZHAO ZHENGTING</creatorcontrib><creatorcontrib>JIANG NING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU YATING</au><au>ZHANG JUNLEI</au><au>XIA YUQI</au><au>LIU DI</au><au>GE XIAOTONG</au><au>XIA ZHENYUAN</au><au>ZHENG XIANTAO</au><au>ZHAO ZHENGTING</au><au>JIANG NING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>LAMP (loop-mediated isothermal amplification) detection primer, detection method and kit for Yunnan tobacco leaf curl virus</title><date>2023-12-05</date><risdate>2023</risdate><abstract>The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) detection primer, a detection method and a kit for Yunnan tobacco leaf curl virus, and the detection primer is TbL-3, TbL-4 or TbL-5. When the obtained primer is used for detecting the LAMP of the Yunnan tobacco leaf curl virus, the sensitivity is 10000 times that of common PCR, the LAMP amplification reaction time is 60 min, the detection efficiency is high, the obtained result can be visually observed and read out, the detection efficiency is effectively improved, and the primer is especially suitable for detection of large-batch samples.
本申请公开了一种云南烟草曲叶病毒用LAMP检测引物、检测方法及其试剂盒，其中检测引物，为：TbL-3、TbL-4或TbL-5。所得引物用于云南烟草曲叶病毒检测LAMP时，灵敏度是普通PCR的10000倍，LAMP扩增反应时间为60min，检测效率较高，所得结果可直观观察读出，有效提高检测效率，尤其适用于大批量样品的检测。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | LAMP (loop-mediated isothermal amplification) detection primer, detection method and kit for Yunnan tobacco leaf curl virus |
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