Kit for detecting swine influenza virus through real-time fluorescence RT-PCR (reverse transcription-polymerase chain reaction)
The invention belongs to the technical field of gene detection, and discloses a real-time fluorescent RT-PCR (reverse transcription-polymerase chain reaction) detection kit for swine influenza virus, which comprises a primer, a specific fluorescent probe, an RNA (ribonucleic acid) extraction reagent...
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| creator | ZHANG XIAOHAN WANG LIN GAO ZHIQIANG REN TONG PU JING XU LIWEI LIU PENGPENG DU SILE LI ZHUO ZHAO XIANGPENG BAI ZILONG LAI PING'AN ZHANG WEI SHI XIJU ZHONG YAN |
| description | The invention belongs to the technical field of gene detection, and discloses a real-time fluorescent RT-PCR (reverse transcription-polymerase chain reaction) detection kit for swine influenza virus, which comprises a primer, a specific fluorescent probe, an RNA (ribonucleic acid) extraction reagent, an RT reaction reagent, a negative control, a positive control, a virus load quantitative standard substance, a data cloud platform, a fluorescent RT-PCR reaction liquid and a specification. The detection method provided by the invention overcomes the problems of long time consumption, low sensitivity, low safety coefficient, poor anti-pollution capability, inaccurate quantification and the like of a conventional detection technology, is rapid and efficient in detection, does not cause cross contamination of samples, and achieves the purpose of simultaneously monitoring sows and piglets. The detection method disclosed by the invention is high in accuracy, high in sensitivity, strong in specificity and excellent i |
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The detection method provided by the invention overcomes the problems of long time consumption, low sensitivity, low safety coefficient, poor anti-pollution capability, inaccurate quantification and the like of a conventional detection technology, is rapid and efficient in detection, does not cause cross contamination of samples, and achieves the purpose of simultaneously monitoring sows and piglets. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Kit for detecting swine influenza virus through real-time fluorescence RT-PCR (reverse transcription-polymerase chain reaction) |
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