Kit and method for amplifying target nucleic acid

The invention relates to a kit and a method for amplifying target nucleic acid. The kit comprises a first specific primer, a first universal primer, a second universal primer, a flanking target fragment, polymerase and nucleotide, the method comprises the following steps: providing a first specific primer, a first universal primer, a second universal primer, a flanking target fragment, polymerase and nucleotide; performing target screening, including a round of first thermal cycle process including a denaturation step, an annealing step and an extension step; and performing amplification comprising two or more rounds of a second thermal cycle process comprising a denaturation step, an annealing step, and an extension step, thereby amplifying the target nucleic acid. The kit and the method for amplifying the target nucleic acid can enhance the specificity of PCR (Polymerase Chain Reaction). 本发明涉及一种扩增靶核酸的试剂盒和方法，该试剂盒包括第一特异性引物，第一通用引物，第二通用引物，侧翼靶片段，聚合酶和核苷酸；该方法包括：提供第一特异性引物，第一通用引物，第二通用引物，侧翼靶片段，聚合酶和核苷酸；进行靶筛选，包括一轮第一热循环