Fusion protein, plasmid and application of fusion protein and plasmid in HIV protease inhibitor screening and efficacy evaluation

The invention provides a fusion protein, a plasmid and application of the fusion protein and the plasmid in HIV protease inhibitor screening and efficacy evaluation. An enzyme cutting substrate sequence of HIV protease is inserted into a gene sequence of the wild type GFP fluorescent protein, and the HIV fluorescent reporter protein obtained after expression has excitation fluorescence activity which is the same as or similar to that of the wild type GFP fluorescent protein. The HIV protease is cloned and expressed on the plasmid of the HIV fluorescence report protein to obtain the fusion protein, the fluorescence effect of the fusion protein can be utilized to detect the activity of the HIV protein and the activity of the HIV protease inhibitor, and an HIV protease inhibitor screening and efficacy evaluation platform can be formed. 本发明提供一种融合蛋白、质粒及其在HIV蛋白酶抑制剂筛选及药效评价中的应用。通过在野生型GFP荧光蛋白的基因序列中插入与HIV蛋白酶的酶切底物序列，经表达后得到的HIV荧光报告蛋白具有与野生型GFP荧光蛋白相同或相近的激发荧光活性。在HIV荧光报告蛋白的质粒上克隆表达HIV蛋白酶，得到融合蛋白，可利用其荧光作用检测HIV蛋白的活性以及HIV蛋白酶抑制剂活性