Method for enhancing embryogenicity of slash pine embryogenicity callus with embryogenicity degeneration
The invention discloses a method for enhancing embryogenicity of slash pine embryogenicity callus with embryogenicity degeneration, and belongs to the field of plant tissue culture. According to the method for enhancing the embryogenic property of the slash pine embryogenic callus with the degenerat...
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| creator | LIU QIAN HU SHAN YANG CHUNXIA GU ZHENJUN DU QIANG DENG ZHAOLEI ZHOU GUANG DING WEI |
| description | The invention discloses a method for enhancing embryogenicity of slash pine embryogenicity callus with embryogenicity degeneration, and belongs to the field of plant tissue culture. According to the method for enhancing the embryogenic property of the slash pine embryogenic callus with the degenerative embryogenic property, the slash pine embryogenic callus with the degenerative embryogenic property is inoculated into a proliferation culture medium for proliferation culture, and the slash pine callus with the enhanced embryogenic property is obtained. The formula of the proliferation culture medium preferably comprises an mLP basal culture medium, 30 g/L of sucrose, 4.0 g/L of gellan gum, 0.5-1.0 mg/L of 6-BA, 0.5-1.0 mg/L of KT, 1.0-2.0 mg/L of NAA, 450 mg/L of L-glutamine, 500 mg/L of casein hydrolysate, 500 mg/L of inositol, 250 mg/L of 2-(N-morpholine) ethanesulfonic acid monohydrate and 0.10 mg/L of IBA. And when the concentration of IBA is 0.10 mg/L, the embryogenic recovery effect of the callus is opti |
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According to the method for enhancing the embryogenic property of the slash pine embryogenic callus with the degenerative embryogenic property, the slash pine embryogenic callus with the degenerative embryogenic property is inoculated into a proliferation culture medium for proliferation culture, and the slash pine callus with the enhanced embryogenic property is obtained. The formula of the proliferation culture medium preferably comprises an mLP basal culture medium, 30 g/L of sucrose, 4.0 g/L of gellan gum, 0.5-1.0 mg/L of 6-BA, 0.5-1.0 mg/L of KT, 1.0-2.0 mg/L of NAA, 450 mg/L of L-glutamine, 500 mg/L of casein hydrolysate, 500 mg/L of inositol, 250 mg/L of 2-(N-morpholine) ethanesulfonic acid monohydrate and 0.10 mg/L of IBA. 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According to the method for enhancing the embryogenic property of the slash pine embryogenic callus with the degenerative embryogenic property, the slash pine embryogenic callus with the degenerative embryogenic property is inoculated into a proliferation culture medium for proliferation culture, and the slash pine callus with the enhanced embryogenic property is obtained. The formula of the proliferation culture medium preferably comprises an mLP basal culture medium, 30 g/L of sucrose, 4.0 g/L of gellan gum, 0.5-1.0 mg/L of 6-BA, 0.5-1.0 mg/L of KT, 1.0-2.0 mg/L of NAA, 450 mg/L of L-glutamine, 500 mg/L of casein hydrolysate, 500 mg/L of inositol, 250 mg/L of 2-(N-morpholine) ethanesulfonic acid monohydrate and 0.10 mg/L of IBA. 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According to the method for enhancing the embryogenic property of the slash pine embryogenic callus with the degenerative embryogenic property, the slash pine embryogenic callus with the degenerative embryogenic property is inoculated into a proliferation culture medium for proliferation culture, and the slash pine callus with the enhanced embryogenic property is obtained. The formula of the proliferation culture medium preferably comprises an mLP basal culture medium, 30 g/L of sucrose, 4.0 g/L of gellan gum, 0.5-1.0 mg/L of 6-BA, 0.5-1.0 mg/L of KT, 1.0-2.0 mg/L of NAA, 450 mg/L of L-glutamine, 500 mg/L of casein hydrolysate, 500 mg/L of inositol, 250 mg/L of 2-(N-morpholine) ethanesulfonic acid monohydrate and 0.10 mg/L of IBA. And when the concentration of IBA is 0.10 mg/L, the embryogenic recovery effect of the callus is opti</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
| title | Method for enhancing embryogenicity of slash pine embryogenicity callus with embryogenicity degeneration |
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