Celery protoplast as well as preparation method and application thereof

The invention discloses a celery protoplast as well as a preparation method and application thereof, and belongs to the field of protoplast preparation. The celery protoplast is obtained by taking tender celery leaves as materials, performing enzymolysis in enzymatic hydrolysate containing cellulase, pectinase and mannitol and then performing centrifugation. The preparation method of the celery protoplast is optimized, 2.0% of cellulase and 0.1% of pectinase are subjected to enzymolysis in an enzymatic hydrolysate containing 0.6 M of mannitol for 8 h, the centrifugal speed of 200 * g is selected, a large amount of high-activity celery protoplast is successfully prepared, an efficient and stable celery protoplast instantaneous conversion system is established, and the high-activity celery protoplast is obtained. Subcellular localization of celery genes is successfully realized, and strong support is provided for celery gene function research and gene editing. 本发明公开了一种芹菜原生质体及其制备方法和应用，属于原生质体制备领域。所述芹菜原生质体以芹菜幼嫩叶片为