Enterococcus faecium WK2 and bacteriocin gene and application thereof

The invention provides enterococcus faecium WK2 as well as a bacteriocin gene and application thereof, and belongs to the technical field of microorganism application, and the collection number of the enterococcus faecium WK2 is CGMCC No.25370. Two bacteriocin genes entB and entX2 obtained from ente...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	LI LIJIE, HAI XURAN, GU YUE, SU SHAOFENG, WU JING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention provides enterococcus faecium WK2 as well as a bacteriocin gene and application thereof, and belongs to the technical field of microorganism application, and the collection number of the enterococcus faecium WK2 is CGMCC No.25370. Two bacteriocin genes entB and entX2 obtained from enterococcus faecium WK2 are optimized and then successfully construct a recombinant vector with an expression plasmid pET28a-EGFP through molecular biology, and the recombinant vector is introduced into a competent cell to obtain a recombinant strain; the recombinant strain can express bacteriocin and has an inhibition effect on Listeria monocytogenes. The enterococcus faecium WK2 has a good antibacterial effect on listeria monocytogenes, escherichia coli, staphylococcus aureus and salmonella. The bacteriocin crude extract extracted by taking enterococcus faecium WK2 fermentation liquor as a raw material has a very remarkable inhibition effect on listeria monocytogenes in milk at different temperatures. 本发明提供了一种屎肠球菌WK