Nostoc punctiforme-derived phenylalanine ammonialyase mutant and application thereof
The invention provides a nostoc punctiforme-derived phenylalanine ammonialyase mutant and application thereof, and belongs to the technical field of biological medicines. The invention provides a nostoc punctiforme-derived phenylalanine ammonialyase mutant. On the basis of NpPAL with an amino acid s...
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| creator | LYU XUDONG WEI XIYU LYU RUIJIE YANG ZHAOYONG JIN YUANYUAN ZHANG QIAN FENG CUIYUE FAN SHUAI ZOU SEN |
| description | The invention provides a nostoc punctiforme-derived phenylalanine ammonialyase mutant and application thereof, and belongs to the technical field of biological medicines. The invention provides a nostoc punctiforme-derived phenylalanine ammonialyase mutant. On the basis of NpPAL with an amino acid sequence as shown in SEQ ID NO: 1, at least one of the following mutations is carried out: 5-10 amino acids at the N tail end are correspondingly mutated into 5-10 amino acids at the N tail end of ApPAL, N2K mutation, I3T mutation and T4L mutation. The recombinant expression protein collected through escherichia coli recombinant expression is a mutant, and compared with NpPAL, the mutant has the advantages that the activity of enzymatic catalysis of phenylalanine into cinnamic acid is greatly improved, meanwhile, the yield of soluble expression is also greatly improved, and a new thought is provided for preparation of drugs for treating phenylketonuria.
本发明提供了一种点形念珠藻来源的苯丙氨酸解氨酶突变体及其应用，属于生物医药技术领域。本发明提供了一种点形念珠藻来源的苯丙氨酸解 |
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本发明提供了一种点形念珠藻来源的苯丙氨酸解氨酶突变体及其应用，属于生物医药技术领域。本发明提供了一种点形念珠藻来源的苯丙氨酸解</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231027&DB=EPODOC&CC=CN&NR=116949025A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231027&DB=EPODOC&CC=CN&NR=116949025A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LYU XUDONG</creatorcontrib><creatorcontrib>WEI XIYU</creatorcontrib><creatorcontrib>LYU RUIJIE</creatorcontrib><creatorcontrib>YANG ZHAOYONG</creatorcontrib><creatorcontrib>JIN YUANYUAN</creatorcontrib><creatorcontrib>ZHANG QIAN</creatorcontrib><creatorcontrib>FENG CUIYUE</creatorcontrib><creatorcontrib>FAN SHUAI</creatorcontrib><creatorcontrib>ZOU SEN</creatorcontrib><title>Nostoc punctiforme-derived phenylalanine ammonialyase mutant and application thereof</title><description>The invention provides a nostoc punctiforme-derived phenylalanine ammonialyase mutant and application thereof, and belongs to the technical field of biological medicines. The invention provides a nostoc punctiforme-derived phenylalanine ammonialyase mutant. On the basis of NpPAL with an amino acid sequence as shown in SEQ ID NO: 1, at least one of the following mutations is carried out: 5-10 amino acids at the N tail end are correspondingly mutated into 5-10 amino acids at the N tail end of ApPAL, N2K mutation, I3T mutation and T4L mutation. The recombinant expression protein collected through escherichia coli recombinant expression is a mutant, and compared with NpPAL, the mutant has the advantages that the activity of enzymatic catalysis of phenylalanine into cinnamic acid is greatly improved, meanwhile, the yield of soluble expression is also greatly improved, and a new thought is provided for preparation of drugs for treating phenylketonuria.
本发明提供了一种点形念珠藻来源的苯丙氨酸解氨酶突变体及其应用，属于生物医药技术领域。本发明提供了一种点形念珠藻来源的苯丙氨酸解</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyj0KwkAQBtA0FqLeYTxAwPgHKSUoVqnSh2HzhSzszizZiZDb23gAq9e8bdG1mk0dpUWc-VHniHLA7D8YKE2QNXBg8QLiGFU8h5UzKC7GYsQyEKcUvGPzKmQTZui4LzYjh4zDz11xfD275l0iaY-c2EFgfdNW1b2-1qfz7XH553wBPng6DA</recordid><startdate>20231027</startdate><enddate>20231027</enddate><creator>LYU XUDONG</creator><creator>WEI XIYU</creator><creator>LYU RUIJIE</creator><creator>YANG ZHAOYONG</creator><creator>JIN YUANYUAN</creator><creator>ZHANG QIAN</creator><creator>FENG CUIYUE</creator><creator>FAN SHUAI</creator><creator>ZOU SEN</creator><scope>EVB</scope></search><sort><creationdate>20231027</creationdate><title>Nostoc punctiforme-derived phenylalanine ammonialyase mutant and application thereof</title><author>LYU XUDONG ; WEI XIYU ; LYU RUIJIE ; YANG ZHAOYONG ; JIN YUANYUAN ; ZHANG QIAN ; FENG CUIYUE ; FAN SHUAI ; ZOU SEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116949025A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LYU XUDONG</creatorcontrib><creatorcontrib>WEI XIYU</creatorcontrib><creatorcontrib>LYU RUIJIE</creatorcontrib><creatorcontrib>YANG ZHAOYONG</creatorcontrib><creatorcontrib>JIN YUANYUAN</creatorcontrib><creatorcontrib>ZHANG QIAN</creatorcontrib><creatorcontrib>FENG CUIYUE</creatorcontrib><creatorcontrib>FAN SHUAI</creatorcontrib><creatorcontrib>ZOU SEN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LYU XUDONG</au><au>WEI XIYU</au><au>LYU RUIJIE</au><au>YANG ZHAOYONG</au><au>JIN YUANYUAN</au><au>ZHANG QIAN</au><au>FENG CUIYUE</au><au>FAN SHUAI</au><au>ZOU SEN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Nostoc punctiforme-derived phenylalanine ammonialyase mutant and application thereof</title><date>2023-10-27</date><risdate>2023</risdate><abstract>The invention provides a nostoc punctiforme-derived phenylalanine ammonialyase mutant and application thereof, and belongs to the technical field of biological medicines. The invention provides a nostoc punctiforme-derived phenylalanine ammonialyase mutant. On the basis of NpPAL with an amino acid sequence as shown in SEQ ID NO: 1, at least one of the following mutations is carried out: 5-10 amino acids at the N tail end are correspondingly mutated into 5-10 amino acids at the N tail end of ApPAL, N2K mutation, I3T mutation and T4L mutation. The recombinant expression protein collected through escherichia coli recombinant expression is a mutant, and compared with NpPAL, the mutant has the advantages that the activity of enzymatic catalysis of phenylalanine into cinnamic acid is greatly improved, meanwhile, the yield of soluble expression is also greatly improved, and a new thought is provided for preparation of drugs for treating phenylketonuria.
本发明提供了一种点形念珠藻来源的苯丙氨酸解氨酶突变体及其应用，属于生物医药技术领域。本发明提供了一种点形念珠藻来源的苯丙氨酸解</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Nostoc punctiforme-derived phenylalanine ammonialyase mutant and application thereof |
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