SSR (Simple Sequence Repeat) primer for identifying 19 chromosomes of poplar and application of SSR primer in breeding primary trisomes of poplar
The invention discloses SSR primers for identifying 19 chromosomes of a poplar and application of the SSR primers in breeding primary trisomes of the poplar. 19 pairs of specific SSR primers which are stable in amplification band, good in polymorphism and capable of identifying and covering 19 chrom...
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| creator | SANG YARU ZHOU QING ZHAO YIFAN KONG BO MA LEXUN DU JIAHUA WU JIAN CHENG XUETONG ZHANG PINGDONG |
| description | The invention discloses SSR primers for identifying 19 chromosomes of a poplar and application of the SSR primers in breeding primary trisomes of the poplar. 19 pairs of specific SSR primers which are stable in amplification band, good in polymorphism and capable of identifying and covering 19 chromosomes of the poplar are screened from 180 pairs of SSR primers, and the 19 pairs of primers can only amplify alleles of the corresponding chromosomes, so that the 19 pairs of primers can be applied to aneuploid offspring group chromosome identification and number identification. According to the method, aneuploidy pollen and normal haploidy female gametes are obtained by utilizing the extreme imbalance separated from homologous chromosomes in the meiosis process of triploid poplar pollen mother cells, pollination hybridization is performed on the aneuploidy pollen and the normal haploidy female gametes to establish aneuploidy offspring groups, and then the number of chromosomes of aneuploidy offspring individuals |
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According to the method, aneuploidy pollen and normal haploidy female gametes are obtained by utilizing the extreme imbalance separated from homologous chromosomes in the meiosis process of triploid poplar pollen mother cells, pollination hybridization is performed on the aneuploidy pollen and the normal haploidy female gametes to establish aneuploidy offspring groups, and then the number of chromosomes of aneuploidy offspring individuals</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231017&DB=EPODOC&CC=CN&NR=116891906A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231017&DB=EPODOC&CC=CN&NR=116891906A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SANG YARU</creatorcontrib><creatorcontrib>ZHOU QING</creatorcontrib><creatorcontrib>ZHAO YIFAN</creatorcontrib><creatorcontrib>KONG BO</creatorcontrib><creatorcontrib>MA LEXUN</creatorcontrib><creatorcontrib>DU JIAHUA</creatorcontrib><creatorcontrib>WU JIAN</creatorcontrib><creatorcontrib>CHENG XUETONG</creatorcontrib><creatorcontrib>ZHANG PINGDONG</creatorcontrib><title>SSR (Simple Sequence Repeat) primer for identifying 19 chromosomes of poplar and application of SSR primer in breeding primary trisomes of poplar</title><description>The invention discloses SSR primers for identifying 19 chromosomes of a poplar and application of the SSR primers in breeding primary trisomes of the poplar. 19 pairs of specific SSR primers which are stable in amplification band, good in polymorphism and capable of identifying and covering 19 chromosomes of the poplar are screened from 180 pairs of SSR primers, and the 19 pairs of primers can only amplify alleles of the corresponding chromosomes, so that the 19 pairs of primers can be applied to aneuploid offspring group chromosome identification and number identification. 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According to the method, aneuploidy pollen and normal haploidy female gametes are obtained by utilizing the extreme imbalance separated from homologous chromosomes in the meiosis process of triploid poplar pollen mother cells, pollination hybridization is performed on the aneuploidy pollen and the normal haploidy female gametes to establish aneuploidy offspring groups, and then the number of chromosomes of aneuploidy offspring individuals</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | SSR (Simple Sequence Repeat) primer for identifying 19 chromosomes of poplar and application of SSR primer in breeding primary trisomes of poplar |
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