Tissue culture, rapid propagation, rooting and transplanting method for lonicera syringae
The invention discloses a lonicera syringae tissue culture rapid propagation rooting and transplanting method, which comprises the following steps: loading vermiculite into 1/3 part of a culture box, adding a nutrient solution, and sterilizing to obtain a rooting substrate; transferring the tissue c...
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| creator | WANG WEI CHEN LUQIN KANG HAO ZHANG JINGYI NAN GUANGYAO CAO JIANTING ZHAO YULIN BAO LONGYU MA JUN REN BAOQING |
| description | The invention discloses a lonicera syringae tissue culture rapid propagation rooting and transplanting method, which comprises the following steps: loading vermiculite into 1/3 part of a culture box, adding a nutrient solution, and sterilizing to obtain a rooting substrate; transferring the tissue culture seedlings into a rooting medium, and culturing for 25-35 days at the temperature of 22-25 DEG C, the illumination intensity of 1800-2500Lux and the photoperiod of 16h/d; when the root length of the seedlings is 1-2 cm, a cover of the culture box is replaced with a cover with a plurality of air holes, the seedlings are transferred into a greenhouse to be subjected to seedling hardening, the seedling hardening environment is that the temperature is 22 +/-2 DEG C, the humidity is 60-65%, and the seedling hardening time is 15 d; after 15 days, the seedlings with the rooting medium are directly transplanted into a hole tray containing the medium, the whole hole tray is wrapped with a preservative film, holes are |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED FISHING FORESTRY HORTICULTURE HUMAN NECESSITIES HUNTING TRAPPING WATERING |
| title | Tissue culture, rapid propagation, rooting and transplanting method for lonicera syringae |
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