Kit for detecting HPV (human papillomavirus) through urine sample
The invention provides a kit for detecting HPV through a urine sample, and relates to the technical field of in-vitro diagnosis. The kit for detecting the HPV through the urine sample comprises a sample nucleic acid releasing agent, an LAMP (Loop-Mediated Isothermal Amplification) reagent and a colo...
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| creator | WEN SUZHEN CHEN HUAYUN YUAN TIANLONG LI QIUHUA TANG BIN |
| description | The invention provides a kit for detecting HPV through a urine sample, and relates to the technical field of in-vitro diagnosis. The kit for detecting the HPV through the urine sample comprises a sample nucleic acid releasing agent, an LAMP (Loop-Mediated Isothermal Amplification) reagent and a color developing agent. The sample nucleic acid releasing agent can release nucleic acid in a urine sample for LAMP isothermal amplification reaction, the color developing agent can monitor the change of the yield of nucleic acid after amplification reaction, and the pH value of the system can change rapidly at a constant temperature due to rapid amplification reaction, so that the detection result is accurate. The color of the reaction reagent is changed from purple red to yellow along with the increase of the amplification product nucleic acid, so as to determine whether the nucleic acid amplification reaction occurs or not and whether the target DNA exists or not. Urine self-sampling has the advantages of being simp |
| format | Patent |
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The kit for detecting the HPV through the urine sample comprises a sample nucleic acid releasing agent, an LAMP (Loop-Mediated Isothermal Amplification) reagent and a color developing agent. The sample nucleic acid releasing agent can release nucleic acid in a urine sample for LAMP isothermal amplification reaction, the color developing agent can monitor the change of the yield of nucleic acid after amplification reaction, and the pH value of the system can change rapidly at a constant temperature due to rapid amplification reaction, so that the detection result is accurate. The color of the reaction reagent is changed from purple red to yellow along with the increase of the amplification product nucleic acid, so as to determine whether the nucleic acid amplification reaction occurs or not and whether the target DNA exists or not. 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The kit for detecting the HPV through the urine sample comprises a sample nucleic acid releasing agent, an LAMP (Loop-Mediated Isothermal Amplification) reagent and a color developing agent. The sample nucleic acid releasing agent can release nucleic acid in a urine sample for LAMP isothermal amplification reaction, the color developing agent can monitor the change of the yield of nucleic acid after amplification reaction, and the pH value of the system can change rapidly at a constant temperature due to rapid amplification reaction, so that the detection result is accurate. The color of the reaction reagent is changed from purple red to yellow along with the increase of the amplification product nucleic acid, so as to determine whether the nucleic acid amplification reaction occurs or not and whether the target DNA exists or not. Urine self-sampling has the advantages of being simp</abstract><oa>free_for_read</oa></addata></record> |
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| language | chi ; eng |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Kit for detecting HPV (human papillomavirus) through urine sample |
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