Preparation method of oligopeptide Fmoc-L-Ala-L-Ala-OH
The invention discloses a preparation method of short peptide Fmoc-L-Ala-L-Ala-OH, which adopts a liquid phase synthesis method and comprises the following steps: reacting N-fluorenylmethoxycarbonyl-L-alanine, namely Fmoc-L-Ala-OH, with an activator HOR in an organic solvent in the presence of a con...
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| creator | XIE BO YANG DACHENG SUN MENGCHUN YANG QIANG WU HUIFENG NAKAYOSHI ZHENG ZHENG ZHANG DONGHAI YANG ZAIKUAN YAO BO |
| description | The invention discloses a preparation method of short peptide Fmoc-L-Ala-L-Ala-OH, which adopts a liquid phase synthesis method and comprises the following steps: reacting N-fluorenylmethoxycarbonyl-L-alanine, namely Fmoc-L-Ala-OH, with an activator HOR in an organic solvent in the presence of a condensing agent to prepare active ester Fmoc-L-Ala-OR, coupling the active ester Fmoc-L-Ala-OR with trimethylsilyl, namely L-alanine, namely H-L-Ala-OH which is temporarily protected by TMS (Trimethylsilyl), and carrying out a reaction to obtain the short peptide Fmoc-L-Ala-L-Ala-OH. And the oligopeptide Fmoc-L-Ala-L-Ala-OH is prepared by the steps of carrying out According to the preparation method,-TMS temporary protection is creatively used for oligopeptide synthesis, the target oligopeptide with the purity higher than 90% can be simply and efficiently prepared without column chromatography purification, and the preparation method has the advantages of being short in step, easy to operate, high in product purity, |
| format | Patent |
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And the oligopeptide Fmoc-L-Ala-L-Ala-OH is prepared by the steps of carrying out According to the preparation method,-TMS temporary protection is creatively used for oligopeptide synthesis, the target oligopeptide with the purity higher than 90% can be simply and efficiently prepared without column chromatography purification, and the preparation method has the advantages of being short in step, easy to operate, high in product purity,</description><language>chi ; eng</language><subject>CHEMISTRY ; METALLURGY ; ORGANIC CHEMISTRY ; PEPTIDES</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230922&DB=EPODOC&CC=CN&NR=116789731A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230922&DB=EPODOC&CC=CN&NR=116789731A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>XIE BO</creatorcontrib><creatorcontrib>YANG DACHENG</creatorcontrib><creatorcontrib>SUN MENGCHUN</creatorcontrib><creatorcontrib>YANG QIANG</creatorcontrib><creatorcontrib>WU HUIFENG</creatorcontrib><creatorcontrib>NAKAYOSHI</creatorcontrib><creatorcontrib>ZHENG ZHENG</creatorcontrib><creatorcontrib>ZHANG DONGHAI</creatorcontrib><creatorcontrib>YANG ZAIKUAN</creatorcontrib><creatorcontrib>YAO BO</creatorcontrib><title>Preparation method of oligopeptide Fmoc-L-Ala-L-Ala-OH</title><description>The invention discloses a preparation method of short peptide Fmoc-L-Ala-L-Ala-OH, which adopts a liquid phase synthesis method and comprises the following steps: reacting N-fluorenylmethoxycarbonyl-L-alanine, namely Fmoc-L-Ala-OH, with an activator HOR in an organic solvent in the presence of a condensing agent to prepare active ester Fmoc-L-Ala-OR, coupling the active ester Fmoc-L-Ala-OR with trimethylsilyl, namely L-alanine, namely H-L-Ala-OH which is temporarily protected by TMS (Trimethylsilyl), and carrying out a reaction to obtain the short peptide Fmoc-L-Ala-L-Ala-OH. And the oligopeptide Fmoc-L-Ala-L-Ala-OH is prepared by the steps of carrying out According to the preparation method,-TMS temporary protection is creatively used for oligopeptide synthesis, the target oligopeptide with the purity higher than 90% can be simply and efficiently prepared without column chromatography purification, and the preparation method has the advantages of being short in step, easy to operate, high in product purity,</description><subject>CHEMISTRY</subject><subject>METALLURGY</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDALKEotSCxKLMnMz1PITS3JyE9RyE9TyM_JTM8vSC0oyUxJVXDLzU_W9dF1zEmEkv4ePAysaYk5xam8UJqbQdHNNcTZQze1ID8-tbggMTk1L7Uk3tnP0NDM3MLS3NjQ0ZgYNQAkeyxE</recordid><startdate>20230922</startdate><enddate>20230922</enddate><creator>XIE BO</creator><creator>YANG DACHENG</creator><creator>SUN MENGCHUN</creator><creator>YANG QIANG</creator><creator>WU HUIFENG</creator><creator>NAKAYOSHI</creator><creator>ZHENG ZHENG</creator><creator>ZHANG DONGHAI</creator><creator>YANG ZAIKUAN</creator><creator>YAO BO</creator><scope>EVB</scope></search><sort><creationdate>20230922</creationdate><title>Preparation method of oligopeptide Fmoc-L-Ala-L-Ala-OH</title><author>XIE BO ; YANG DACHENG ; SUN MENGCHUN ; YANG QIANG ; WU HUIFENG ; NAKAYOSHI ; ZHENG ZHENG ; ZHANG DONGHAI ; YANG ZAIKUAN ; YAO BO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116789731A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>CHEMISTRY</topic><topic>METALLURGY</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><toplevel>online_resources</toplevel><creatorcontrib>XIE BO</creatorcontrib><creatorcontrib>YANG DACHENG</creatorcontrib><creatorcontrib>SUN MENGCHUN</creatorcontrib><creatorcontrib>YANG QIANG</creatorcontrib><creatorcontrib>WU HUIFENG</creatorcontrib><creatorcontrib>NAKAYOSHI</creatorcontrib><creatorcontrib>ZHENG ZHENG</creatorcontrib><creatorcontrib>ZHANG DONGHAI</creatorcontrib><creatorcontrib>YANG ZAIKUAN</creatorcontrib><creatorcontrib>YAO BO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>XIE BO</au><au>YANG DACHENG</au><au>SUN MENGCHUN</au><au>YANG QIANG</au><au>WU HUIFENG</au><au>NAKAYOSHI</au><au>ZHENG ZHENG</au><au>ZHANG DONGHAI</au><au>YANG ZAIKUAN</au><au>YAO BO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Preparation method of oligopeptide Fmoc-L-Ala-L-Ala-OH</title><date>2023-09-22</date><risdate>2023</risdate><abstract>The invention discloses a preparation method of short peptide Fmoc-L-Ala-L-Ala-OH, which adopts a liquid phase synthesis method and comprises the following steps: reacting N-fluorenylmethoxycarbonyl-L-alanine, namely Fmoc-L-Ala-OH, with an activator HOR in an organic solvent in the presence of a condensing agent to prepare active ester Fmoc-L-Ala-OR, coupling the active ester Fmoc-L-Ala-OR with trimethylsilyl, namely L-alanine, namely H-L-Ala-OH which is temporarily protected by TMS (Trimethylsilyl), and carrying out a reaction to obtain the short peptide Fmoc-L-Ala-L-Ala-OH. And the oligopeptide Fmoc-L-Ala-L-Ala-OH is prepared by the steps of carrying out According to the preparation method,-TMS temporary protection is creatively used for oligopeptide synthesis, the target oligopeptide with the purity higher than 90% can be simply and efficiently prepared without column chromatography purification, and the preparation method has the advantages of being short in step, easy to operate, high in product purity,</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | CHEMISTRY METALLURGY ORGANIC CHEMISTRY PEPTIDES |
| title | Preparation method of oligopeptide Fmoc-L-Ala-L-Ala-OH |
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