Biomarker combination, kit and system for predicting lymph node metastasis of bladder cancer

The invention provides a biomarker combination and a kit for predicting lymph node metastasis of bladder cancer. The biomarker combination comprises MESP1 mRNA (Messenger Ribonucleic Acid), EFEMP1 mRNA, PIGZ mRNA, KRT23 mRNA and CALML3 mRNA. Specific qPCR (quantitative polymerase chain reaction) primers are respectively designed aiming at mRNA (messenger ribonucleic acid) of five genes, and the score of a biomarker combination is calculated by detecting the expression level of each biomarker in biopsy tumor tissues. The invention further provides a system for predicting bladder cancer lymph node metastasis, the system shows good accuracy, sensitivity and specificity, the lymph node state can be rapidly and accurately evaluated in the early stage, and reference can be provided for accurate treatment of bladder cancer patients. 本发明提供了一种用于预测膀胱癌淋巴结转移的生物标记物组合、试剂盒，所述生物标记物组合包括MESP1mRNA、EFEMP1mRNA、PIGZ mRNA、KRT23mRNA和CALML3mRNA。本发明针对5个基因的mRNA分别设计特异性qPCR引物，通过在活检肿瘤组织中检测生物各标记物的表达水平，计算出生物标记物组合的评分。本发明还提供了一种用于预测膀胱癌淋巴结转移的系统