Fusion gene related paraffin embedded section reference, primer, probe and preparation method

The invention provides a fusion gene related paraffin embedded section reference, a primer, a probe and a preparation method thereof, and the fusion gene related paraffin embedded section reference is obtained by adopting a partial artificial synthesis mode to supplement lacked raw materials and com...

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Hauptverfasser: WANG JUN, ZHANG WEI, XU MEIBO, ZHANG SHUO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a fusion gene related paraffin embedded section reference, a primer, a probe and a preparation method thereof, and the fusion gene related paraffin embedded section reference is obtained by adopting a partial artificial synthesis mode to supplement lacked raw materials and combining with human immortalized cells. The stable multi-site mixed RNA fusion gene paraffin embedding section reference can be obtained, the stability is high, and the conditions such as storage and transportation are simple; the multi-site RNA fusion gene paraffin embedding section reference product integrates a plurality of RNA fusion genes, and has the advantages of realizing detection of a plurality of sites in one detection system, saving reagents, saving time and the like; the absolute copy number quantification is carried out by adopting the digital PCR technology, and the copy value of the reference is adjusted to the same level, so that the absolute constant value data of the copy number of each fusion gene