Bee-derived modified antibacterial peptide AC-7 as well as preparation and application thereof

The invention discloses a bee source modified antibacterial peptide AC-7 as well as preparation and application thereof. The bee source modified antibacterial peptide AC-7 is obtained by modifying the amino acid sequence of the antibacterial peptide Abaecin. Compared with the antibacterial peptide Abaecin, the antibacterial peptide AC-7 has the advantages that the amphipathicity is relatively high, and the quantity of electric charges is relatively large. Bacteriostatic experiments show that the antibacterial peptide AC-7 has a strong and broad-spectrum antibacterial effect, the inhibiting effect on gram-negative bacteria is stable, the effective MIC is 2-4 mu g/mL, and the MIC value of gram-positive bacteria is 4-16 mu g/mL. 本发明公开了一种蜂源改造体抗菌肽AC-7及其制备和应用。所述蜂源改造体抗菌肽AC-7是根据抗菌肽Abaecin的氨基酸序列经过改造获得的。相较于抗菌肽Abaecin，抗菌肽AC-7具有较高的两亲性且带有较多的电荷量。抑菌实验表明，抗菌肽AC-7具有强效广谱的抑菌效果，对革兰氏阴性菌的抑制效果稳定且有效MIC为2~4μg/mL，革兰氏阳性菌MIC值为4~16μg/mL。