Application of different doses of mesenchymal stem cells to cartilage cell differentiation and proliferation

The invention discloses an application of different doses of mesenchymal stem cells to cartilage cell differentiation and proliferation, which comprises the following steps: separation and amplification: separating and obtaining mesenchymal stem cells from human or animal in-vivo or in-vitro tissues; dose selection: selecting different cell densities or quantities; carrying out cell treatment, and incubating mesenchymal stem cells and cartilage cells; cell detection: detecting cell differentiation indexes through real-time PCR (polymerase chain reaction), immunofluorescence, immunoblotting and other technologies; cell evaluation: evaluating the processed cartilage cells to understand the influence of mesenchymal stem cells on differentiation and proliferation of the cartilage cells; and data analysis: according to an evaluation result, comparing differences between different dose groups and between the dose groups and a control group. According to the invention, a series of mesenchymal stem cells with differe