Endotoxin affinity chromatography filler, preparation method, chromatographic column and filtering equipment

Endotoxin affinity chromatography filler, preparation method, chromatographic column and filtering equipment

The invention discloses endotoxin affinity chromatography packing, a preparation method, a chromatographic column and filtering equipment, the preparation method comprises the following steps: dissolving cellulose by using a sodium hydroxide/thiourea aqueous solution green solvent, preparing cellulo...

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Hauptverfasser: MU NING, RUAN BIWU, SUN GUOWEI
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Sprache:chi ; eng
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CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
THEIR RELEVANT APPARATUS
TRANSPORTING
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creator MU NING
RUAN BIWU
SUN GUOWEI
description The invention discloses endotoxin affinity chromatography packing, a preparation method, a chromatographic column and filtering equipment, the preparation method comprises the following steps: dissolving cellulose by using a sodium hydroxide/thiourea aqueous solution green solvent, preparing cellulose microspheres by an emulsification method, taking epichlorohydrin as an activator, connecting hexamethylenediamine ligand, and preparing the endotoxin affinity chromatography packing by using the hydrophobic effect of an alkyl chain of hexamethylenediamine and a lipid A region. Affinity adsorption is carried out under the charge effect of amino, and the endotoxin removal effect is good. 本申请公开了内毒素亲和色谱填料、制备方法、色谱柱及过滤设备，所述制备方法以氢氧化钠/硫脲水溶液绿色溶剂溶解纤维素，通过乳化法制备纤维素微球，以表氯醇作为活化剂，接己二胺配基，利用己二胺的烷基链与脂质A区的疏水作用，以及氨基的电荷作用进行亲和吸附，去除内毒素效果佳。
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subjects CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
THEIR RELEVANT APPARATUS
TRANSPORTING
title Endotoxin affinity chromatography filler, preparation method, chromatographic column and filtering equipment
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