Kit and method for simultaneously detecting aedes albopictus, Zika virus, Chikungunya virus and dengue virus based on RAA technology

The invention relates to a kit and a method for simultaneously detecting aedes albopictus, Zika virus, Chikungunya virus and dengue virus based on RAA technology. According to the present invention, the recombinase-mediated strand displacement nucleic acid amplification technology of the mitochondri...

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Hauptverfasser:	YANG YU, LIU DI, YU YINGQUAN, TENG XINDONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a kit and a method for simultaneously detecting aedes albopictus, Zika virus, Chikungunya virus and dengue virus based on RAA technology. According to the present invention, the recombinase-mediated strand displacement nucleic acid amplification technology of the mitochondrial DNA cytochrome oxidase I gene, the Zika virus NS5 gene, the Chikungunya virus NS2 gene and the dengue virus NS1 gene is used for mosquito identification and mosquito-carried pathogen detection; mosquito molecule identification efficiency and pathogen detection efficiency can be greatly improved. 本发明涉及一种基于RAA技术的白纹伊蚊及携带寨卡病毒、基孔肯雅病毒和登革病毒同时检测试剂盒及检测方法。本发明依据线粒体DNA细胞色素氧化酶I基因、寨卡病毒NS5基因、基孔肯雅病毒的NS2基因、登革病毒的NS1基因的重组酶介导链置换核酸扩增技术应用于蚊类鉴定和蚊类携带病原体检测，与传统的形态学鉴定方法和荧光PCR方法相比，可大大提高蚊类分子鉴定效率和病原体检测效率。