Method for preparing staphylococcus aureus pneumonia mouse model and model evaluation
The invention discloses a method for preparing a staphylococcus aureus pneumonia mouse model and model evaluation. The method comprises the following steps: step 1, marking staphylococcus aureus in vitro; step 2, establishment of a staphylococcus aureus pneumonia mouse model: by adopting a mode of e...
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| creator | LIU FEI LIAO CHANGBO TANG YING CHEN SIJIE JIAO YONG FANG LIBEN |
| description | The invention discloses a method for preparing a staphylococcus aureus pneumonia mouse model and model evaluation. The method comprises the following steps: step 1, marking staphylococcus aureus in vitro; step 2, establishment of a staphylococcus aureus pneumonia mouse model: by adopting a mode of exposing a trachea and atomizing bacteria by a quantitative aerosol atomizing device in the trachea of the mouse through an oral trachea, the amount inhaled by the mouse can be controlled at the same level, the error of each group is smaller, and the modeling quality is higher; the lung imaging condition of the mouse is observed in vivo through a near-infrared imaging system, the mouse is successfully modeled when the mouse lung has an obvious and uniform near-infrared fluorescence image, whether the model is successfully established or not can be known at the first time, the hysteresis is avoided, and the modeling success rate can be effectively improved. And the evaluation method does not need a series of invasive |
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The method comprises the following steps: step 1, marking staphylococcus aureus in vitro; step 2, establishment of a staphylococcus aureus pneumonia mouse model: by adopting a mode of exposing a trachea and atomizing bacteria by a quantitative aerosol atomizing device in the trachea of the mouse through an oral trachea, the amount inhaled by the mouse can be controlled at the same level, the error of each group is smaller, and the modeling quality is higher; the lung imaging condition of the mouse is observed in vivo through a near-infrared imaging system, the mouse is successfully modeled when the mouse lung has an obvious and uniform near-infrared fluorescence image, whether the model is successfully established or not can be known at the first time, the hysteresis is avoided, and the modeling success rate can be effectively improved. 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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PHYSICS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TESTING TRAPPING VINEGAR WINE |
| title | Method for preparing staphylococcus aureus pneumonia mouse model and model evaluation |
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