Detection kit for largemouth bass iridovirus egg yolk antibody and detection method thereof

The invention discloses a detection kit for a largemouth bass iridovirus egg yolk antibody and a detection method of the largemouth bass iridovirus egg yolk antibody. The ELISA detection method of the largemouth bass iridovirus egg yolk antibody provided by the invention is simple to operate, recombinant expression protein does not need to be constructed, the inactivated largemouth bass iridovirus is directly used for optimizing detection conditions, and the common problems of poor specificity, low sensitivity and the like of the detection method are solved; besides, the method is short in time consumption, can obtain a detection result within one day, is suitable for monitoring the reduction level of the antibody in different immune stages in the laying hen immune process in real time, is beneficial to solving the problem of in-vitro titer evaluation of the iridovirus egg yolk antibody, provides guidance for screening of an iridovirus egg yolk antibody immune procedure, and has a good application prospect. A