Strawberry fusarium oxysporum PCR (Polymerase Chain Reaction) detection primer and application thereof

The invention discloses a strawberry fusarium oxysporum PCR (Polymerase Chain Reaction) detection primer and application thereof. The nucleotide sequences of the detection primer are shown as SEQ ID NO: 1 and SEQ ID NO: 2 in a sequence table. The primer pair disclosed by the invention has good specificity and can be used as a primer for detecting fusarium oxysporum; a verification experiment is carried out after DNA extraction of field soil and laboratory seedlings, and a result shows that the pair of specific primers has application feasibility. The experiment has important research values in the aspects of early warning of seedling diseases, detection of soil pathogens, tracking and monitoring of diseases in the growth period of strawberries and the like, and has certain application prospects in evaluation of the disinfection effect of strawberry greenhouse soil and detection of fusarium oxysporum of strawberry seedlings. 本发明公开了草莓尖孢镰刀菌PCR检测引物及其应用。所述检测引物的核苷酸序列如序列表SEQ ID NO：1和SEQ ID NO：2所示。本发明的对引物具有良好的特异性，可以作