RNAi primer group, recombinant expression vector, transformant, kit and method for regulating and controlling Frankliniella occidentalis
The invention discloses an RNAi primer group, a recombinant expression vector, a transformant, a kit and a method for regulating and controlling Frankliniella occidentalis, and belongs to the field of molecular biology. The RNAi primer group is selected from one pair, two pairs or three pairs of the...
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| creator | YUAN JIANGJIANG QIAN KANGHUA TANG YINGXI TOYOHISA AKIRA WU QINGJUN ZHENG XIAOBIN |
| description | The invention discloses an RNAi primer group, a recombinant expression vector, a transformant, a kit and a method for regulating and controlling Frankliniella occidentalis, and belongs to the field of molecular biology. The RNAi primer group is selected from one pair, two pairs or three pairs of the following primer pairs: a FoTRE1-1 primer pair of which the upstream and downstream primer sequences are respectively shown as SEQ ID NO.1 and SEQ ID NO.2; the upstream primer sequence and the downstream primer sequence of the FoTRE1-2 primer pair are respectively as shown in SEQ ID NO.3 and SEQ ID NO.4; the upstream primer sequence and the downstream primer sequence of the FoTRE2 primer pair are shown as SEQ ID NO.5 and SEQ ID NO.6 respectively. After a recombinant expression vector formed by amplification products of the three primer pairs of the primer group is transferred into a target plant, the egg laying rate and the hatching rate of the Frankliniella occidentalis eating the target plant can be remarkably r |
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The RNAi primer group is selected from one pair, two pairs or three pairs of the following primer pairs: a FoTRE1-1 primer pair of which the upstream and downstream primer sequences are respectively shown as SEQ ID NO.1 and SEQ ID NO.2; the upstream primer sequence and the downstream primer sequence of the FoTRE1-2 primer pair are respectively as shown in SEQ ID NO.3 and SEQ ID NO.4; the upstream primer sequence and the downstream primer sequence of the FoTRE2 primer pair are shown as SEQ ID NO.5 and SEQ ID NO.6 respectively. 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The RNAi primer group is selected from one pair, two pairs or three pairs of the following primer pairs: a FoTRE1-1 primer pair of which the upstream and downstream primer sequences are respectively shown as SEQ ID NO.1 and SEQ ID NO.2; the upstream primer sequence and the downstream primer sequence of the FoTRE1-2 primer pair are respectively as shown in SEQ ID NO.3 and SEQ ID NO.4; the upstream primer sequence and the downstream primer sequence of the FoTRE2 primer pair are shown as SEQ ID NO.5 and SEQ ID NO.6 respectively. 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The RNAi primer group is selected from one pair, two pairs or three pairs of the following primer pairs: a FoTRE1-1 primer pair of which the upstream and downstream primer sequences are respectively shown as SEQ ID NO.1 and SEQ ID NO.2; the upstream primer sequence and the downstream primer sequence of the FoTRE1-2 primer pair are respectively as shown in SEQ ID NO.3 and SEQ ID NO.4; the upstream primer sequence and the downstream primer sequence of the FoTRE2 primer pair are shown as SEQ ID NO.5 and SEQ ID NO.6 respectively. After a recombinant expression vector formed by amplification products of the three primer pairs of the primer group is transferred into a target plant, the egg laying rate and the hatching rate of the Frankliniella occidentalis eating the target plant can be remarkably r</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | RNAi primer group, recombinant expression vector, transformant, kit and method for regulating and controlling Frankliniella occidentalis |
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