Complete system for breeding anti-TGEV infection pig variety and application thereof

The invention discloses a complete system for breeding a pig variety resistant to swine transmissible gastroenteritis virus infection and application of the complete system. The system comprises a gene editing protein, pAPN-sgRNA-1, pAPN-sgRNA-2 and a donor DNA, the gene editing protein can perform effective enzyme digestion on two target sites of a pAPN gene, a segment to be subjected to point-fixed modification between the two target sites is replaced with the donor DNA, a codon for coding chromogenic acid at the 737th site of the pig pAPN protein can be mutated into a codon for coding alanine, and the gene editing protein can be used for carrying out site-fixed modification on the segment to be subjected to point-fixed modification on the segment to be subjected to point-fixed modification on the segment to be subjected to point-fixed modification on the segment to be subjected to point-fixed modification on the segment to be subjected to point-fixed modification. Further, accurate mutation of the 737th am