SNP (Single Nucleotide Polymorphism) site primer combination for Wugengfeng chicken resource identification and application of SNP site primer combination
The invention discloses a set of SNP (Single Nucleotide Polymorphism) site primer combination for identifying WMFO (Wugu Guangdong) chicken resources and application of the SNP site primer combination. The primer combination is used for amplifying any two to five of WMF1 sites to WMF5 sites. When th...
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| creator | LUO KEYIN LIU YIFAN TU YUNJIE SHENG ZHONGWEI ZOU JIANMIN WANG XIANKE JU XIAOJUN JI GAIGE SHU JINGTING ZHANG MING WANG GUANJI SHAN YANJU XIAO QIN |
| description | The invention discloses a set of SNP (Single Nucleotide Polymorphism) site primer combination for identifying WMFO (Wugu Guangdong) chicken resources and application of the SNP site primer combination. The primer combination is used for amplifying any two to five of WMF1 sites to WMF5 sites. When the primer combination disclosed by the invention is used for identifying the Wumongolian chicken resources, as long as the genotype of one SNP (Single Nucleotide Polymorphism) site does not conform to the corresponding genotype of the Wumongolian chicken, the individual can be completely excluded from belonging to the Wumongolian chicken; any two SNP sites are selected for combination, the judgment probability of conforming to the genotype of the UMongolian Fengfeng chicken can reach 97% or above, and all five SNP sites are combined, and the judgment probability of conforming to the genotype of the UMongolian Fengfeng chicken can reach 100%. The identification method is simple and convenient to operate, high in accu |
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The primer combination is used for amplifying any two to five of WMF1 sites to WMF5 sites. When the primer combination disclosed by the invention is used for identifying the Wumongolian chicken resources, as long as the genotype of one SNP (Single Nucleotide Polymorphism) site does not conform to the corresponding genotype of the Wumongolian chicken, the individual can be completely excluded from belonging to the Wumongolian chicken; any two SNP sites are selected for combination, the judgment probability of conforming to the genotype of the UMongolian Fengfeng chicken can reach 97% or above, and all five SNP sites are combined, and the judgment probability of conforming to the genotype of the UMongolian Fengfeng chicken can reach 100%. 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The primer combination is used for amplifying any two to five of WMF1 sites to WMF5 sites. When the primer combination disclosed by the invention is used for identifying the Wumongolian chicken resources, as long as the genotype of one SNP (Single Nucleotide Polymorphism) site does not conform to the corresponding genotype of the Wumongolian chicken, the individual can be completely excluded from belonging to the Wumongolian chicken; any two SNP sites are selected for combination, the judgment probability of conforming to the genotype of the UMongolian Fengfeng chicken can reach 97% or above, and all five SNP sites are combined, and the judgment probability of conforming to the genotype of the UMongolian Fengfeng chicken can reach 100%. 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The primer combination is used for amplifying any two to five of WMF1 sites to WMF5 sites. When the primer combination disclosed by the invention is used for identifying the Wumongolian chicken resources, as long as the genotype of one SNP (Single Nucleotide Polymorphism) site does not conform to the corresponding genotype of the Wumongolian chicken, the individual can be completely excluded from belonging to the Wumongolian chicken; any two SNP sites are selected for combination, the judgment probability of conforming to the genotype of the UMongolian Fengfeng chicken can reach 97% or above, and all five SNP sites are combined, and the judgment probability of conforming to the genotype of the UMongolian Fengfeng chicken can reach 100%. The identification method is simple and convenient to operate, high in accu</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | SNP (Single Nucleotide Polymorphism) site primer combination for Wugengfeng chicken resource identification and application of SNP site primer combination |
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