Tissue culture and rapid propagation method of eucalyptus citrina

The invention discloses a tissue culture and rapid propagation method of eucalyptus nigra, which comprises the following steps: taking leaves of the eucalyptus nigra and branches of the eucalyptus nigra as explants, selecting newly-expanded tender leaves and newly-grown branches, the explants of the...

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Hauptverfasser: HUANG RENZE, ZHOU XIAOYI, HUANG XIN, PANG HUIDAN, QUAN GUODONG, HUANG QING, ZHOU BAOBIAO, WEN JUN, ZHONG RIMEI, ZENG YUECHUAN, LI NANNAN, YE YANJUN, XU XIAOHUI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a tissue culture and rapid propagation method of eucalyptus nigra, which comprises the following steps: taking leaves of the eucalyptus nigra and branches of the eucalyptus nigra as explants, selecting newly-expanded tender leaves and newly-grown branches, the explants of the two groups of branches are newly grown, the pollutants are less, the failure rate of the experiment can be reduced, and in addition, the explants of the branches of the eucalyptus nigra can be used as explants of the leaves of the eucalyptus nigra and the branches of the eucalyptus nigra. The growth function of the selected tender leaves and branches is vigorous, and the two groups of tender leaves and branches are inoculated into the culture medium in the later period; the method comprises the following steps: disinfecting an explant, washing the explant with tap water, draining water, cutting leaves and branches into the size of 1cm * 1cm, and culturing. According to the tissue culture and rapid propagation meth