Bovine whole genome-based CRISPR-Cas9 knockout library, knockout cell bank and target gene screening method
The invention provides a bovine whole genome CRISPR-Cas9-based knockout library, a knockout cell bank and a method for screening a target gene, and relates to the technical field of library construction. The invention provides a construction method of a cattle whole genome coding gene CRISPR-Cas9 li...
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| creator | LIU BIN DAI LINGLI BAO YANCHUN GUO LILI SHI CAIXIA ZHANG WENGUANG LIU ZAIXIA |
| description | The invention provides a bovine whole genome CRISPR-Cas9-based knockout library, a knockout cell bank and a method for screening a target gene, and relates to the technical field of library construction. The invention provides a construction method of a cattle whole genome coding gene CRISPR-Cas9 library, and on the basis of the constructed cattle whole genome CRISPR/Cas9 knockout library, an MDBK cattle kidney epithelial cell whole genome knockout cell library is constructed for the first time. Based on a cattle whole genome CRISPR/Cas9 knockout library strategy, nine candidate genes related to beta-hydroxybutyric acid (BHBA) action are screened and identified for the first time and can be used as molecular markers for drug screening and marker-assisted management or molecular targets for preparing a gene editing disease-resistant animal model.
本发明提供了基于牛全基因组CRISPR-Cas9敲除文库、敲除细胞库及筛选目标基因的方法，涉及文库构建技术领域。本发明提供了一种牛全基因组编码基因CRISPR-Cas9文库的构建方法，并基于构建得到的牛全基因组CRISPR/Cas9敲除文库，首次构建了MDBK牛肾上皮细胞全基因组敲除细胞库。本发明基于牛全基因组CRISPR/Cas |
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本发明提供了基于牛全基因组CRISPR-Cas9敲除文库、敲除细胞库及筛选目标基因的方法，涉及文库构建技术领域。本发明提供了一种牛全基因组编码基因CRISPR-Cas9文库的构建方法，并基于构建得到的牛全基因组CRISPR/Cas9敲除文库，首次构建了MDBK牛肾上皮细胞全基因组敲除细胞库。本发明基于牛全基因组CRISPR/Cas</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMBINATORIAL CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230630&DB=EPODOC&CC=CN&NR=116356431A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230630&DB=EPODOC&CC=CN&NR=116356431A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU BIN</creatorcontrib><creatorcontrib>DAI LINGLI</creatorcontrib><creatorcontrib>BAO YANCHUN</creatorcontrib><creatorcontrib>GUO LILI</creatorcontrib><creatorcontrib>SHI CAIXIA</creatorcontrib><creatorcontrib>ZHANG WENGUANG</creatorcontrib><creatorcontrib>LIU ZAIXIA</creatorcontrib><title>Bovine whole genome-based CRISPR-Cas9 knockout library, knockout cell bank and target gene screening method</title><description>The invention provides a bovine whole genome CRISPR-Cas9-based knockout library, a knockout cell bank and a method for screening a target gene, and relates to the technical field of library construction. The invention provides a construction method of a cattle whole genome coding gene CRISPR-Cas9 library, and on the basis of the constructed cattle whole genome CRISPR/Cas9 knockout library, an MDBK cattle kidney epithelial cell whole genome knockout cell library is constructed for the first time. Based on a cattle whole genome CRISPR/Cas9 knockout library strategy, nine candidate genes related to beta-hydroxybutyric acid (BHBA) action are screened and identified for the first time and can be used as molecular markers for drug screening and marker-assisted management or molecular targets for preparing a gene editing disease-resistant animal model.
本发明提供了基于牛全基因组CRISPR-Cas9敲除文库、敲除细胞库及筛选目标基因的方法，涉及文库构建技术领域。本发明提供了一种牛全基因组编码基因CRISPR-Cas9文库的构建方法，并基于构建得到的牛全基因组CRISPR/Cas9敲除文库，首次构建了MDBK牛肾上皮细胞全基因组敲除细胞库。本发明基于牛全基因组CRISPR/Cas</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMBINATORIAL CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjLEKwkAQBdNYiPoPa2-KEA2k1EPRRiTah81lTcJddsPdqfj3Kgi2Vg-GmTeOzEbuHRM8WrEEDbH0FFfoqQZVHM6nIlboczAs2sgtgO0qh-65-BFN1kKFbAC5hoCuofA5IvDaEXHHDfQUWqmn0eiK1tPsu5Novtte1D6mQUryA-p3FUp1TJIsXWXLNFmn_zgvGltAOA</recordid><startdate>20230630</startdate><enddate>20230630</enddate><creator>LIU BIN</creator><creator>DAI LINGLI</creator><creator>BAO YANCHUN</creator><creator>GUO LILI</creator><creator>SHI CAIXIA</creator><creator>ZHANG WENGUANG</creator><creator>LIU ZAIXIA</creator><scope>EVB</scope></search><sort><creationdate>20230630</creationdate><title>Bovine whole genome-based CRISPR-Cas9 knockout library, knockout cell bank and target gene screening method</title><author>LIU BIN ; DAI LINGLI ; BAO YANCHUN ; GUO LILI ; SHI CAIXIA ; ZHANG WENGUANG ; LIU ZAIXIA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116356431A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMBINATORIAL CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU BIN</creatorcontrib><creatorcontrib>DAI LINGLI</creatorcontrib><creatorcontrib>BAO YANCHUN</creatorcontrib><creatorcontrib>GUO LILI</creatorcontrib><creatorcontrib>SHI CAIXIA</creatorcontrib><creatorcontrib>ZHANG WENGUANG</creatorcontrib><creatorcontrib>LIU ZAIXIA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU BIN</au><au>DAI LINGLI</au><au>BAO YANCHUN</au><au>GUO LILI</au><au>SHI CAIXIA</au><au>ZHANG WENGUANG</au><au>LIU ZAIXIA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Bovine whole genome-based CRISPR-Cas9 knockout library, knockout cell bank and target gene screening method</title><date>2023-06-30</date><risdate>2023</risdate><abstract>The invention provides a bovine whole genome CRISPR-Cas9-based knockout library, a knockout cell bank and a method for screening a target gene, and relates to the technical field of library construction. The invention provides a construction method of a cattle whole genome coding gene CRISPR-Cas9 library, and on the basis of the constructed cattle whole genome CRISPR/Cas9 knockout library, an MDBK cattle kidney epithelial cell whole genome knockout cell library is constructed for the first time. Based on a cattle whole genome CRISPR/Cas9 knockout library strategy, nine candidate genes related to beta-hydroxybutyric acid (BHBA) action are screened and identified for the first time and can be used as molecular markers for drug screening and marker-assisted management or molecular targets for preparing a gene editing disease-resistant animal model.
本发明提供了基于牛全基因组CRISPR-Cas9敲除文库、敲除细胞库及筛选目标基因的方法，涉及文库构建技术领域。本发明提供了一种牛全基因组编码基因CRISPR-Cas9文库的构建方法，并基于构建得到的牛全基因组CRISPR/Cas9敲除文库，首次构建了MDBK牛肾上皮细胞全基因组敲除细胞库。本发明基于牛全基因组CRISPR/Cas</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Bovine whole genome-based CRISPR-Cas9 knockout library, knockout cell bank and target gene screening method |
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