Kit for detecting Kawasaki disease related CCR7 gene methylation degree and application thereof
The invention discloses a kit for detecting the methylation degree of a Kawasaki disease related CCR7 gene. The kit comprises a pair of CCR7 gene promoter region two CG site methylation specific amplification primers and a methylation specific sequencing primer, a nucleotide sequence of an upstream...
Gespeichert in:
Hauptverfasser: | , , , , , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention discloses a kit for detecting the methylation degree of a Kawasaki disease related CCR7 gene. The kit comprises a pair of CCR7 gene promoter region two CG site methylation specific amplification primers and a methylation specific sequencing primer, a nucleotide sequence of an upstream primer of the methylation specific amplification primer is as shown in SEQ ID NO.1: 5 '-Biotin-TAATGTTTTTAGAGATAAAGGGGTAGAAGA-3', a nucleotide sequence of a downstream primer of the methylation specific amplification primer is as shown in SEQ ID NO.2, and a nucleotide sequence of a downstream primer of the methylation specific amplification primer is as shown in SEQ ID NO.3 '. The nucleotide sequence of a downstream primer of the methylation specific amplification primer is shown as SEQ ID NO.2: 5 '-ACACATCTATAACTCCAACA-3', and the nucleotide sequence of the methylation specific sequencing primer is shown as SEQ ID NO.3: 5 '-CCTCATATAATTCTACCA-3'. The kit is used for auxiliary diagnosis of the Kawasaki disease, dru |
---|