Kit for detecting Kawasaki disease related CCR7 gene methylation degree and application thereof

The invention discloses a kit for detecting the methylation degree of a Kawasaki disease related CCR7 gene. The kit comprises a pair of CCR7 gene promoter region two CG site methylation specific amplification primers and a methylation specific sequencing primer, a nucleotide sequence of an upstream...

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Bibliographische Detailangaben
Hauptverfasser: ZHU FENGSHENG, HUANG LIXIN, SHEN YIJING, QIU HAIYAN, ZHENG BANGXU, WU JUNHUA, SHEN YU, LU WEIHUI, YU BEIRONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a kit for detecting the methylation degree of a Kawasaki disease related CCR7 gene. The kit comprises a pair of CCR7 gene promoter region two CG site methylation specific amplification primers and a methylation specific sequencing primer, a nucleotide sequence of an upstream primer of the methylation specific amplification primer is as shown in SEQ ID NO.1: 5 '-Biotin-TAATGTTTTTAGAGATAAAGGGGTAGAAGA-3', a nucleotide sequence of a downstream primer of the methylation specific amplification primer is as shown in SEQ ID NO.2, and a nucleotide sequence of a downstream primer of the methylation specific amplification primer is as shown in SEQ ID NO.3 '. The nucleotide sequence of a downstream primer of the methylation specific amplification primer is shown as SEQ ID NO.2: 5 '-ACACATCTATAACTCCAACA-3', and the nucleotide sequence of the methylation specific sequencing primer is shown as SEQ ID NO.3: 5 '-CCTCATATAATTCTACCA-3'. The kit is used for auxiliary diagnosis of the Kawasaki disease, dru