On-site collection method of karst plateau lake water environment DNA sample

The invention discloses an on-site collection method of a karst plateau lake water environment DNA sample, which comprises the following steps: step 1, a worker needs to wear disposable sterile gloves before sampling, and a water sampler and a plastic barrel are fully rinsed and washed 3-5 times at a sampling point; 2, collecting a water sample by using a water collector, pouring the water sample into a washed plastic barrel, filling 2 L of water into a sterile wide-mouth bottle, and then immediately carrying out low-temperature dark storage treatment; 3, carrying out suction filtration on the water sample by using a diaphragm vacuum pump and a sand core glass filtering device; and 4, after the suction filtration work is completed, enriching the eDNA in the water sample on the filter membrane, rolling up the filter membrane by using tweezers, placing the rolled filter membrane in a sterile centrifugal tube, and storing the rolled filter membrane at-20 DEG C. The invention provides the sampling method of the w