Method for improving flux of unstable protein in ultrafiltration process

The invention discloses a method for reducing protein precipitation generated in the ultrafiltration process of a TNF-alpha monoclonal antibody, the method comprises affinity chromatography, anion chromatography, cation chromatography and ultrafiltration, and in the ultrafiltration process, arginine is added in a sample solution and an elution buffer solution. Through the effect of arginine on inhibiting aggregates, hydrogen bonds between proteins are reduced, so that insoluble particles formed by unstable antibody proteins are reduced, and the ultrafiltration flux is improved. 本发明公开了一种减少TNF-α单克隆抗体超滤过程中产生蛋白沉淀的方法，包括亲和层析、阴离子层析、阳离子层析和超滤，所述超滤过程中，在样品溶液和洗脱缓冲液中添加精氨酸。通过精氨酸抑制聚集体的作用，减少蛋白质之间的氢键，从而减少不稳定抗体蛋白形成不溶性微粒，提高超滤通量。