Improved CTAB method for extracting nucleic acid of paulownia fordii

The invention belongs to the field of molecular ecology experiments, and particularly provides a CTAB (cetyltrimethyl ammonium bromide) extraction method for an improved genome DNA (deoxyribonucleic acid) version of an endangered semi-mangrove plant tung paulownia. The endangered semi-mangrove plant...

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Hauptverfasser: NI LIANG, TAN ZUOLI, YU JING, ZHANG SHIJIE, WANG YONG, ZHANG XIUHAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of molecular ecology experiments, and particularly provides a CTAB (cetyltrimethyl ammonium bromide) extraction method for an improved genome DNA (deoxyribonucleic acid) version of an endangered semi-mangrove plant tung paulownia. The endangered semi-mangrove plant paulownia fortunei is rich in polysaccharide and polyphenol, so that the tissue grinding fluid is extremely viscous, and the extraction of nucleic acid with good quality is extremely difficult. On the basis of a traditional CTAB method, cells are split by adopting an improved lysis solution, and extraction is carried out through a mixed solution of Tris balanced phenol-chloroform-isoamyl alcohol in a certain proportion. And precipitating the DNA by using sodium acetate-isopropanol with improved volume, and purifying the DNA, so as to obtain the high-quality genome DNA. The DNA extracted by adopting the method for extracting the nucleic acid of the endangered semi-mangrove plant tung paulownia, provided by the inve